"
Team:Panama/20 July 2010
From 2010.igem.org
Nikkibiotech (Talk | contribs) (→July 20) |
Nikkibiotech (Talk | contribs) (→July 20) |
||
| Line 7: | Line 7: | ||
'''Methodology Steps:''' | '''Methodology Steps:''' | ||
| - | 1.Locate the BioBrick parts that we are going to assemble, in the distribution plates. | + | '''1.Locate the BioBrick parts that we are going to assemble, in the distribution plates.''' |
RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N | RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N | ||
| Line 13: | Line 13: | ||
For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C. | For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C. | ||
| - | 2.Clone the chosen BioBrick parts in competent ''E. coli''. | + | '''2.Clone the chosen BioBrick parts in competent ''E. coli''.''' |
For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids & prepared LB-ampi. For the ampicilin we have to make a frozen stock solution with a concentration of 50ug/ml in water. The plate concentration is of 100mg/ml. | For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids & prepared LB-ampi. For the ampicilin we have to make a frozen stock solution with a concentration of 50ug/ml in water. The plate concentration is of 100mg/ml. | ||
Revision as of 20:46, 15 October 2010
July 20
Activities:
Methodology Steps:
1.Locate the BioBrick parts that we are going to assemble, in the distribution plates.
RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N
For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C.
2.Clone the chosen BioBrick parts in competent E. coli.
For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids & prepared LB-ampi. For the ampicilin we have to make a frozen stock solution with a concentration of 50ug/ml in water. The plate concentration is of 100mg/ml.
We want to make a stock solution of 2ml so, we need 100mg of ampicilin to make 50mg/ml. We have to filter this through a 0.2um filter.
|
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
