Team:Panama/20 August 2010

(Difference between revisions)

Revision as of 14:11, 26 October 2010

iGEM Panama

Restriction reactions

Today the work was focused on the digestion reaction of three parts, RBS, Promotor and the plasmid.

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2) RBS

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3) Plasmid

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For the sample visualization, we performed an electrophosis on a agarose gel.

@@ Line 15: / Line 15: @@
-) RBS
-Buffer 2 ------------> 0.5ul
-BSA -----------------> 5ul
-Xba -----------------> 1ul
+) RBS
+[[Image:Cuadro20agostoRBS.JPG|200px|thumb|left|alt text]]
-Pst1 ----------------> 1ul
-DNA()-------> 20ul
-H2O -----------------> 22.5ul
-Final volume = 50ul.
 ) Plasmid
-Buffer 2 ------------> 0,5  ul
+[[Image:Cuadro20agostoplasmid.JPG|200px|thumb|left|alt text]]
-BSA -----------------> 5    ul
-EcoR1----------------> 1    ul
-Spel ----------------> 1    ul
-DNA(BBa_psB1A2)------> 20   ul
-H2O -----------------> 22,5 ul
-Final volume= 50 ul.