Team:Panama/19 October 2010
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PCR of Rh1ab | PCR of Rh1ab | ||
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Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE | Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE | ||
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Revision as of 23:20, 27 October 2010
October 19
PCR of Rh1ab
Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE
We observe bands around 5000bp. It could be the linear plasmid DNA
Sample 4 was the only one that didn't present bands
PCR: to amplify RH1ab, samples of plasmid DNA DNAp=2ul Primer 1= 1 ul Primer 2= 1 ul Master Mix= 12.5 ul Water= 8.5 ul Total= 25 ul
We inoculated 30 colonies en 3 ml liquid LB medium + Amp at 37C, overnight with shaking
30 white colonies and one blue colony as a negative control
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