Team:Panama/14 October 2010
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October 14
Prepared Ampicillin stock (50 mg/ml)
We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert (Rh1ab) or not.
Miniprep Protocol: For this procedure we used what is called a "dirty miniprep".
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