Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/02

From 2010.igem.org

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You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
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===Transformation===
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===Experiment:Transformation===
'''member'''
'''member'''

Revision as of 07:01, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/10/02 Saturday (watachin)

Experiment:Electrophoresis

member

naoto and easily

material

  • pSB1C3
  • bcsA
  • bcsB
  • bcsC
  • bcsD

all of them had cut with XbaI and SpeI

Result

2010-10-02-02.jpg

Experiment:Ligation

member

easily

material

  • pSB1C3
  • bcsC

You can see other materials at protocol8

procedure

You can see our way of ligation at protocol8

Experiment:Transformation

member

easily

material

  • bcsC in pSB1C3

You can see other materials at protocol9

procedure

You can see our way of ligation at protocol9