Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/02

From 2010.igem.org

(Difference between revisions)
Line 2: Line 2:
==2010/10/02 Saturday (watachin)==
==2010/10/02 Saturday (watachin)==
-
===Electrophoreses of PCR production===
+
===Experiment:Electrophoresis===
 +
'''member'''
 +
 
 +
naoto and easily
 +
 
 +
'''material'''
 +
*pSB1C3
 +
*bcsA
 +
*bcsB
 +
*bcsC
 +
*bcsD
 +
all of them had cut with XbaI and SpeI
 +
 
'''Result'''
'''Result'''
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[[Image:2010-10-02-02.jpg]]
[[Image:2010-10-02-02.jpg]]
-
===PCR===
+
===Experiment:Ligation===
-
===Transformation===
+
'''member'''
 +
 
 +
easily
 +
 
 +
'''material'''
 +
*pSB1C3
 +
*bcsC
 +
You can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
 +
 
 +
'''procedure'''
 +
 
 +
You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
 +
 
 +
===Experiment:Transformation===
 +
'''member'''
 +
 
 +
easily
 +
 
 +
'''material'''
 +
*bcsC in pSB1C3
 +
You can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol9</a></html>
 +
 
 +
'''procedure'''
 +
 
 +
You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol9</a></html>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br/>

Revision as of 16:11, 24 October 2010


E.coli Fiber Project Notebook

August 2010
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Contents

2010/10/02 Saturday (watachin)

Experiment:Electrophoresis

member

naoto and easily

material

  • pSB1C3
  • bcsA
  • bcsB
  • bcsC
  • bcsD

all of them had cut with XbaI and SpeI

Result

2010-10-02-01.jpg

2010-10-02-02.jpg

Experiment:Ligation

member

easily

material

  • pSB1C3
  • bcsC

You can see other materials at protocol8

procedure

You can see our way of ligation at protocol8

Experiment:Transformation

member

easily

material

  • bcsC in pSB1C3

You can see other materials at protocol9

procedure

You can see our way of ligation at protocol9