Team:Bielefeld-Germany/Judging/BioBricks

From 2010.igem.org

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BioBricks

BioBricks are the central figure of the iGEM competition. It is pretty important to construct functional and well characterized BioBricks in order to avoid problems for other users. Unfortunately we had to handle some problems with BioBricks from the database. Although we created a lot of BioBricks, we therefore focused on creating good, functional and well characterized BioBricks. Our best three BioBricks are listed below. The detailed information of these BioBricks can be received by using the links.


K389004

Luciferase from pGL4.10[luc2]:

Partinfo of the BioBrick BBa_K389004

Characterization of K389004


K389015

Final sensor system with luciferase readout:

Partinfo of the BioBrick BBa_K389015

Characterization of K389015


K389016

Final sensor system with mRFP readout:

Partinfo of the BioBrick BBa_K389016

Characterization of K389016


Correction of faulty BioBricks

Initially, we wanted to use the VirA BioBrick BBa_K238008 and the virB promoter BBa_K238011 in our project, but could only obtain unexpected/faulty restriction patterns. Finally we chose to sequence these parts, hoping to find the cause for the maintained restriction patterns. Unfortunately we could not approve the sequences of BBa_K238008 and BBa_K238011 deposited in the partsregistry so that we chose to design our own VirA and virB promoter BioBricks. We strongly recommend using our VirA receptor and virB promoter since it has been approved by multiple means, e.g. restriction patterns and sequencing (BBa_K389001 and BBa_K389003).


Improvement of an existing BioBrick

By sending in our mutated virG gene BBa_K389002 that works in E. coli without the rpoA gene from A. tumefaciens we improved the existing virG BioBrick from the registry BBa_K238009.

Characterizing already existing BioBricks

We helped characterizing the following BioBricks: