Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28

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E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/09/28

1:DNA extraction

<Member>
mizuki,Takahiro

<Sample>
PCR products

  • 1(9/16)
  • 2(9/16)
  • 3(9/17)
  • 4(9/16)
  • 5(9/16)
  • 7(9/16)

<Protocol>
See Protocol 4

2:DNA Ligation

<Member>
mio

<Sample>

(after digestion)

<Protocol>
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 4 (70)
  • 5 (70)
  • 9 (63,68)
  • 10(58)
  • vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

  • 1 (9/28)
  • 2 (9/28)
  • 4 (9/28)
  • 5 (9/28)
  • 8 (9/27)
  • 9 (9/28)
  • 10(9/28)
  • vector

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-28 17hr 31min.JPG

6:DNA extraction

<Member>
Takahiro

<Sample>
PCR products

<Protocol>
See Protocol 4


7:DNA Digestion

<Member>
Mariko

<Sample, Materials>
・PCR productions

  • 1 (8/31)
  • 2 (9/11)
  • 4L(8/26)
  • 5 (8/31)

<Protocol>
See Prptpcpl 9

8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 3 (69)
  • 4 (70)

<Protocol>
See Protocol 1