Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28

From 2010.igem.org

(Difference between revisions)
(New page: ===1：DNA extraction=== ＜Member＞<br /> mizuki,Takahiro ＜Sample＞<br /> PCR products *1(9/16) *2(9/16) *3(9/17) *4(9/16) *5(9/16) *7(9/16) ＜Protocol＞<br /> See [http://2010.ig...)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/28=
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===1：DNA extraction===
===1：DNA extraction===
＜Member＞<br />
＜Member＞<br />
Line 5: Line 9:
＜Sample＞<br />
＜Sample＞<br />
PCR products
PCR products
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*1(9/16)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1](9/16)
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*2(9/16)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2](9/16)
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*3(9/17)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3](9/17)
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*4(9/16)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4](9/16)
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*5(9/16)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](9/16)
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*7(9/16)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](9/16)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===2：DNA Ligation ===
===2：DNA Ligation ===
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＜Sample＞<br />
＜Sample＞<br />
-
*7-8-10,vector
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10],vector
-
*2,vector  
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2],vector  
(after digestion)
(after digestion)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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===3:PCR===
===3:PCR===
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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
-
*1 (72)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (72)
-
*2 (70)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (70)
-
*4 (70)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (70)
-
*5 (70)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (70)
-
*9 (63,68)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (63,68)
-
*10(58)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](58)
*vector(72)
*vector(72)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===4:Transformation===
===4:Transformation===
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<Sample><br />
<Sample><br />
-
*7-8-10+vector
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]+vector
-
*2+vector
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]+vector
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
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===5:Electrophoresis===
===5:Electrophoresis===
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<Sample><br />
<Sample><br />
PCR products
PCR products
-
*1 (9/28)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/28)
-
*2 (9/28)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/28)
-
*4 (9/28)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/28)
-
*5 (9/28)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (9/28)
-
*8 (9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (9/27)
-
*9 (9/28)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (9/28)
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*10(9/28)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](9/28)
*vector
*vector
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<Result><br />
<Result><br />
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[[Image:]]
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[[Image:Matsuura_2010-09-28_17hr_31min.JPG‎]]
-
 
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===6：DNA extraction===
===6：DNA extraction===
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
-
*1 (8/31)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (8/31)
-
*2 (9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/11)
-
*4L(8/26)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4L](8/26)
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*5 (8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (8/31)
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+
-
・Digest enzyme
+
-
*Avr2
+
-
*Nhe1
+
-
*spe1
+
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
-
 
===8:PCR===
===8:PCR===
Line 117: Line 109:
<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
-
*1 (72)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (72)
-
*2 (70)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (70)
-
*3 (69)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] (69)
-
*4 (70)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (70)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]

Latest revision as of 02:54, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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Contents

2010/09/28

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

  • 1(9/16)
  • 2(9/16)
  • 3(9/17)
  • 4(9/16)
  • 5(9/16)
  • 7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 4 (70)
  • 5 (70)
  • 9 (63,68)
  • 10(58)
  • vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

  • 1 (9/28)
  • 2 (9/28)
  • 4 (9/28)
  • 5 (9/28)
  • 8 (9/27)
  • 9 (9/28)
  • 10(9/28)
  • vector

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-28 17hr 31min.JPG

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4


7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/11)
  • 4L(8/26)
  • 5 (8/31)

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 3 (69)
  • 4 (70)

<Protocol>
See Protocol 1

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/28"