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INSA-Lyon/8 September 2010
From 2010.igem.org
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<p>Start of 6 liquid cultur from the plates of PHL1273 of yesterday : <br> | <p>Start of 6 liquid cultur from the plates of PHL1273 of yesterday : <br> | ||
| - | 3 at 28°C and | + | 3 at 28°C and a shaking speed of 200 rpm, 3 at 28°C and a shaking speed of 50rpm</p><br> |
<p>Digestion (with Roche enzyme) : <br> | <p>Digestion (with Roche enzyme) : <br> | ||
<ol id="list_extra"> | <ol id="list_extra"> | ||
| Line 78: | Line 59: | ||
<li>Phasin, 22B (clones 1,2,3), 24M (clones 1,2,3) : X+P</li> | <li>Phasin, 22B (clones 1,2,3), 24M (clones 1,2,3) : X+P</li> | ||
<li>negative witness without enzyme for Curli and Phasin</li> | <li>negative witness without enzyme for Curli and Phasin</li> | ||
| - | </ol><br> | + | </ol><br></p> |
| - | The Curli and Phasin extraction were contaminated and it destroy the DNA during the digestion. We will make new extractions.</p><br> | + | <p>The Curli and Phasin extraction were contaminated and it destroy the DNA during the digestion. We will make new extractions.</p><br> |
<p>Result of the transformation : <br> | <p>Result of the transformation : <br> | ||
<ol id="list_extra" > | <ol id="list_extra" > | ||
| Line 88: | Line 69: | ||
<li>12/13 : </li> | <li>12/13 : </li> | ||
<li>pha A/pha B :</Ii> | <li>pha A/pha B :</Ii> | ||
| - | </ol><br> | + | </ol><br></p> |
| - | Liquid culture of 10 clones of pha A/pha B, 12/13, L4</p><br> | + | <p>Liquid culture of 10 clones of pha A/pha B, 12/13, L4</p><br> |
<p>Culture on plate of Phasin, Curli and 18A/24A </p> | <p>Culture on plate of Phasin, Curli and 18A/24A </p> | ||
<br><br><br><br><br><br><br> | <br><br><br><br><br><br><br> | ||
Latest revision as of 19:54, 25 October 2010
September 8th
Start of 6 liquid cultur from the plates of PHL1273 of yesterday :
3 at 28°C and a shaking speed of 200 rpm, 3 at 28°C and a shaking speed of 50rpm
Digestion (with Roche enzyme) :
- Curli : E+S
- Phasin, 22B (clones 1,2,3), 24M (clones 1,2,3) : X+P
- negative witness without enzyme for Curli and Phasin
The Curli and Phasin extraction were contaminated and it destroy the DNA during the digestion. We will make new extractions.
Result of the transformation :
- 8C/14K : 1 clone
- 18A/24A : 1 clone
- 4O/10L : nothing
- L3/18A (L4) :
- 12/13 :
- pha A/pha B :
Liquid culture of 10 clones of pha A/pha B, 12/13, L4
Culture on plate of Phasin, Curli and 18A/24A
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