INSA-Lyon/14 October 2010

From 2010.igem.org





October 14th


Transformation of the ligation of the 12/10 (with purified DNA)


Liquid cultures of the ligations of the 12/10 (with unpurified DNA) :

  1. phasin/phasin-intein : 4 clones
  2. phasin-intein/2P: 7 clones
  3. phaC/12/13 and Curli/phaC: too much clones, the ligation didn't work
  4. 18A/L2/1N : no clones

Digestion of the clones with X+P








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