INSA-Lyon/28 July 2010

(Difference between revisions)

Latest revision as of 19:33, 25 October 2010

Results of the Transformation of 1F/24A: about a hundred of clones.

We select 10 clones and we make a liquid pre culture with Amp.

New digestion of 1F by S+P and 24A by X+P (extracted on 07/27)

Agarose gel electrophoresis: ok

Extraction and purification of the band 24A with a kit (concentrated in 20µL)

Ligation of 1F/24A: ratio--> 5/10µL

Transformation of the ligation

Transformation of L3 on tetracycline plate

Digestion by NheI of L1

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 <p> Results of the Transformation of 1F/24A: about a hundred of clones.</p>
-<br>
 <p>We select 10 clones and we make a liquid pre culture with Amp. </p>
 <br>
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 <p>Ligation of 1F/24A: ratio--> 5/10µL </p>
 <p> Transformation of the ligation </p>
+ <br>
 <p>Transformation of L3 on tetracycline plate</p>
 <p>Digestion by NheI of L1<br><br></p>