E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/09/23 1.1 1：DNA Digestion 1.2 2:PCR 1.3 3：DNA extraction 1.4 4:Electrophoresis 1.5 5:Electrophoresis 1.6 6：DNA extraction from gels 1.7 7：DNA Digestion

2010/09/23

1：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞
・PCR productions

• 1
• 2
• 4
• 5

＜Protocol＞
See Prptpcpl 9

2:PCR

<Member>
Hitomi

<Sample>(temperature in annealing)

• 7(67.5)
• 9(57,61,69)

<Protocol>
See Protocol 1

3：DNA extraction

＜Member＞
Hitomi

＜Sample＞
PCR products(9/23)

＜Protocol＞
See Protocol 4

4:Electrophoresis

<Member>
Hitomi

<Sample>
Digestion products.

• 1
• 2
• 3
• 4
• 5

<Protocol>
See Protocol 8

<Result>

5:Electrophoresis

<Member>
Hitomi

<Sample>
PCR products (9/23)

<Protocol>
See Protocol 8

6：DNA extraction from gels

＜Member＞
Mariko,Hitomi

＜Sample＞
Digestion products (9/23) (After electrophoresis)

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞

• 2 (9/23)
• 4 (9/23)

＜Protocol＞
See Prptpcpl 9