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From 2010.igem.org

Contents 1 Tested BioBricks 1.1 <partinfo>K238008</partinfo>: virA 1.2 <partinfo>BBa_K238011</partinfo>: vir-promotor 1.3 <partinfo>P1010</partinfo>: ccdB-gene 2 Sequenced BioBricks 2.1 Own BioBricks

Tested BioBricks

We tested the following BioBricks:

<partinfo>K238008</partinfo>: virA

We made a restriction analysis and sequenced parts of this BioBrick. There were problems - more information within a short time.

<partinfo>BBa_K238011</partinfo>: vir-promotor

We made a restriction analysis and sequenced parts of this BioBrick.

<partinfo>P1010</partinfo>: ccdB-gene

We transformed this BioBrick into Escherichia coli JM109, DH5α, TOP10 and DB3.1. E. coli JM109 and DH5α seem to be ccdB resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in E. coli TOP10 (no colonies after transformation) and DB3.1 (lawn after transformation).

Sequenced BioBricks

Own BioBricks

The sequencing of the following of our own BioBricks was succesful and lead to the expected results:

• <partinfo>K389001</partinfo> (not fully completed)

• <partinfo>K389002</partinfo>

• <partinfo>K389003</partinfo>

• <partinfo>K389004</partinfo>

• <partinfo>K389005</partinfo>

• <partinfo>K389010</partinfo> (not fully completed)

• <partinfo>K389012</partinfo> (not fully completed)

• <partinfo>K389013</partinfo> (not fully completed)