Team:INSA-Lyon/Project/Stage3/Strategy

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Strategy


We choose to follow two differents strategies to obtain our systems of regulation.

  1. Thermoregulation

For this system of regulation, we decided to synthesized directly the sequence of the curli promoter with the igem restriction sites. In the same time a part with the gene ompR234 is created by PCR, from the genome of the chassi PHL818. Then we ligate an igem reporter gene with the curli promoter and we wanted to make a double transformation with ompR234 to see if our parts work as we hoped. The final plasmid construction of the thermometer RNA will contain a constitutive promoter(BBa_J23119), the thermometer RNA, the PPI-protein fused and a terminator.


  1. Control under Arabinose

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