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From 2010.igem.org

Helping other teams

Correction of a faulty BioBrick - VirA from Agrobacterium tumefaciens (BBa_K238008), Group: iGEM09_KULeuven

Initially, we wanted to use this part in our project, but could only obtain unexpected/faulty restriction patterns. Finally we chose to sequence the part, hoping to find the cause for the maintained restriction patterns. Unfortunately we could not approve the sequence of <partinfo>BBa_K238008</partinfo> deposited in the partsregistry so that we chose to design our own VirA BioBrick. I strongly recommend using our VirA since it has been approved by multiple means, e.g. restriction patterns and sequencing (<partinfo>K389001</partinfo>).