Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

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E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 14hr 29min.JPG


2:DNA extraction

<Member>
nito

<Sample>

<Protocol>
See Protocol 4


3:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10


4:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


5:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


6:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4


7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


8:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


9:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7

(After electrophoresis)


<Protocol>
SeeProtocol 4


10:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4