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From 2010.igem.org

Contents 1 Introduction 2 Agrobacterium tumefaciens 3 Native receptor 4 Read out system 5 Receptor modifiction strategy 6 Screening system 7 Modeling 8 Weblinks

Introduction

Our iGEM project is to create an E. coli cell which is capable to sense Capsaicin in a complex sample and report the concentration with a luciferase light signal. The native receptor senses Acetosyringone. We used directed evolution to modify the binding region of the native receptor to generate a new capsaicin receptor. We established a screening system based on antibiotic concentration gradients to screen the newly generated receptors. In our E. coli Acetosyringone sensing system several parts driving from different organisms were assemlbed. The native receptor was subcloned from Agrobacterium tumefaciens to create a read out system with firefly luciferase.

Agrobacterium tumefaciens

Native receptor

Read out system

Receptor modifiction strategy

Screening system

Modeling

Weblinks