|
|
Line 1: |
Line 1: |
- | {{Template:INSALyon2009_Test}} | + | <!-- {{INSA-Lyon_style}} --> |
| + | {{INSA-Lyon/stylegroningen}} |
| + | {{INSA-Lyon/header}} |
| + | {{INSA-Lyon/maincontent}} |
| | | |
- | <html>
| + | {{INSA-Lyon/menugauche}} |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | </html>
| + | |
| | | |
- | {{Template:INSALyon2010_Calendar}}
| + | <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en"> |
| | | |
- | <html> | + | <style type="text/css";> |
- | <div style="text-align:justify ; padding: 0 110px 50px 110px"> | + | .firstHeading{background-image: url(https://static.igem.org/mediawiki/2010/9/93/Team_INSA-Lyon_header_DroppyColi.jpg); backgroung-repeat: no repeat;} |
| + | ul#topnav li:hover { |
| + | background: #f200d0 url("https://static.igem.org/mediawiki/2010/d/d9/Team_INSA-Lyon_bouton_fushia.gif") repeat-x; |
| + | } |
| + | ul#topnav li span { background: #f200d0;} |
| + | </style> |
| + | |
| + | <script type="text/javascript"> |
| + | $(document).ready(function() { |
| + | |
| + | $("ul#topnav li").hover(function() { //Hover over event on list item |
| + | $(this).css({ 'background' : '#f200d0 url(https://static.igem.org/mediawiki/2010/d/d9/Team_INSA-Lyon_bouton_fushia.gif) repeat-x'}); //Add background color and image on hovered list item |
| + | $(this).find("span").show(); //Show the subnav |
| + | } , function() { //on hover out... |
| + | $(this).css({ 'background' : 'none'}); //Ditch the background |
| + | $(this).find("span").hide(); //Hide the subnav |
| + | }); |
| + | |
| + | }); |
| + | </script> |
| + | |
| + | <div class="colonnegauche"> |
| + | <div id="menug"> |
| + | <ul> |
| + | <li><a href="/Team:INSA-Lyon/Project/Theory" class="cteal"> > Theory</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/Strategy" class="slateb"> > Strategy</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/Results" class="yellow"> > Results</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li> |
| + | |
| + | <div id="ssmenug"> |
| + | <li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li> |
| + | </div> |
| + | |
| + | <li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modeling</a></li> |
| + | <li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li> |
| + | |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | <div id="corps2"> |
| + | <br> |
| + | <h3>July 12</h3> |
| </html> | | </html> |
| | | |
- | ==July 12==
| + | ---- |
| | | |
| <html> | | <html> |
- |
| |
| <style type="text/css"> | | <style type="text/css"> |
- | #list_extra{list-style-type:none;} | + | #list_extra{list-style-type:none; margin: 10px auto auto 100px;} |
- | #list_extra2{list-style-type:circle;} | + | #list_extra2{list-style-type:circle; margin: 10px auto auto 100px;} |
- | #table1{margin: 10px auto auto 20px; | + | #table1{margin: 10px auto auto 30px; |
| border-collapse: collapse; | | border-collapse: collapse; |
| text-align: center;} | | text-align: center;} |
Line 29: |
Line 71: |
| </style> | | </style> |
| | | |
- | <ul>
| + | |
- | <li>Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161 <br> | + | <p>Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161</p> |
- | <li>Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis <br><br> | + | <p>Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis </p><br><br> |
| <p> | | <p> |
| <img class="image" src="" alt="rajouter la photo de gel" /> | | <img class="image" src="" alt="rajouter la photo de gel" /> |
| </p><br><br> | | </p><br><br> |
- | | + | <p>Digestion: <br></p> |
- | <li>Digestion: <br> | + | |
| | | |
| <table id ="table1"> | | <table id ="table1"> |
Line 80: |
Line 121: |
| <i>no gel picture available</i> | | <i>no gel picture available</i> |
| </ol> | | </ol> |
- | | + | <br><br> |
- | <li>ligation + transformation: (during 3 hours at room temperature)<br> | + | <p>Ligations + transformations: (during 3 hours at room temperature)<br></p> |
| <ol id="list_extra2"> | | <ol id="list_extra2"> |
| <li>BBa_J23119+BBa_K115017 in plasmid pSB1C3 (3A method): constitutive promoter + ARNth | | <li>BBa_J23119+BBa_K115017 in plasmid pSB1C3 (3A method): constitutive promoter + ARNth |
Line 87: |
Line 128: |
| <li>BBa_J23119+BBa_K115017 : constitutive promoter + ARNth | | <li>BBa_J23119+BBa_K115017 : constitutive promoter + ARNth |
| </ol> | | </ol> |
- | </ul>
| |
| | | |
| + | <br><br><br><br><br> |
| + | </html> |
| + | {{Template:INSALyon2010_Calendar}} |
| + | <html> |
| + | </div> |
| | | |
| + | <div id="corps4" style="border:0px;"> |
| | | |
| + | <div style="float:justify;border:solid 0px green;> <!-- mettre 1px si cadre --> |
| + | -moz-border-radius: 15px; |
| + | -webkit-border-radius: 15px; |
| + | border-radius: 15px;"> |
| + | |
| + | <div style="text-align:center;"> |
| + | <a id="csb" href="http://fondation.insa-lyon.fr/"><img style="width: 120px; height: 60px; margin: 10px; padding-top: 15px; padding-bottom: 15px" src="https://static.igem.org/mediawiki/2010/8/81/Team_INSA-Lyon_logo_fondationINSA.jpg"/></a> |
| + | |
| + | <a id="csb" href="http://www.biomerieux.fr/"><img style="width: 120px; height: 90px; margin: 10px" src="http://www.biomerieux.fr/france/img/logo_home.png"/></a> |
| + | |
| + | |
| + | <a id="csb" href="http://www.insa-lyon.fr/en"><img style="width: 120px; height: 64.2px; margin: 15px; padding-top: 10px; padding-bottom: 10px" src="https://static.igem.org/mediawiki/2010/4/46/Team_INSA-Lyon_logo_Insa.png" /></a> |
| + | |
| + | |
| + | </div> |
| </div> | | </div> |
| + | |
| + | </div> |
| + | |
| </html> | | </html> |
July 12
Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161
Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis
Digestion:
part |
enzyme 1 |
enzyme 2 |
BBa_B0015 |
E |
X |
BBa_K115017 |
E |
X |
BBa_K115017 |
X |
P |
BBa_J23119 |
E |
S |
BBa_E0040 |
E |
S |
Ligations + transformations: (during 3 hours at room temperature)
June
|
M | T | W | T | F | S | S
|
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_June_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_June_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_June_2010&action=edit 6]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_June_2010&action=edit 13]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_June_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_June_2010&action=edit 20]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_June_2010&action=edit 26]
| [http://2010.igem.org/INSA-Lyon/27_June_2010 27]
|
[http://2010.igem.org/INSA-Lyon/28_June_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_June_2010 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_June_2010&action=edit 30]
|
|
July
|
M | T | W | T | F | S | S
|
|
|
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_July_2010&action=edit 4]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_July_2010&action=edit 5]
| [http://2010.igem.org/INSA-Lyon/6_July_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_July_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_July_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_July_2010 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_July_2010&action=edit 10]
| [http://2010.igem.org/INSA-Lyon/11_July_2010 11]
|
[http://2010.igem.org/INSA-Lyon/12_July_2010 12]
| [http://2010.igem.org/INSA-Lyon/13_July_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_July_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_July_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_July_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_July_2010 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_July_2010&action=edit 18]
|
[http://2010.igem.org/INSA-Lyon/19_July_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_July_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_July_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_July_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_July_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_July_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_July_2010 25]
|
[http://2010.igem.org/INSA-Lyon/26_July_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_July_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_July_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_July_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_July_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_July_2010 31]
|
|
August
|
M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_August_2010&action=edit 1]
|
[http://2010.igem.org/INSA-Lyon/2_August_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_August_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_August_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_August_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_August_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_August_2010&action=edit 8]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_August_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_August_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_August_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_August_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_August_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_August_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_August_2010&action=edit 15]
|
[http://2010.igem.org/INSA-Lyon/16_August_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_August_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_August_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_August_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_August_2010 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_August_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_August_2010&action=edit 22]
|
[http://2010.igem.org/INSA-Lyon/23_August_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_August_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_August_2010 25]
| [http://2010.igem.org/INSA-Lyon/26_August_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_August_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_August_2010 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_August_2010&action=edit 29]
|
[http://2010.igem.org/INSA-Lyon/30_August_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_August_2010 31]
|
|
September
|
M | T | W | T | F | S | S
|
|
| [http://2010.igem.org/INSA-Lyon/1_September_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_September_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_September_2010&action=edit 5]
|
[http://2010.igem.org/INSA-Lyon/6_September_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_September_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_September_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_September_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_September_2010&action=edit 12]
|
[http://2010.igem.org/INSA-Lyon/13_September_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_September_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_September_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_September_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_September_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_September_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_September_2010 19]
|
[http://2010.igem.org/INSA-Lyon/20_September_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_September_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_September_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_September_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_September_2010 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_September_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_September_2010&action=edit 26]
|
[http://2010.igem.org/INSA-Lyon/27_September_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_September_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_September_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_September_2010 30]
|
|
October
|
M | T | W | T | F | S | S
|
|
|
|
| [http://2010.igem.org/INSA-Lyon/1_October_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_October_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_October_2010 3]
|
[http://2010.igem.org/INSA-Lyon/4_October_2010 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_October_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_October_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_October_2010&action=edit 7]
| [http://2010.igem.org/INSA-Lyon/8_October_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_October_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_October_2010 10]
|
[http://2010.igem.org/INSA-Lyon/11_October_2010 11]
| [http://2010.igem.org/INSA-Lyon/12_October_2010 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_October_2010&action=edit 13]
| [http://2010.igem.org/INSA-Lyon/14_October_2010 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_October_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_October_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_October_2010&action=edit 17]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_October_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_October_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_October_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_October_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_October_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_October_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_October_2010&action=edit 24]
|
[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_October_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_October_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/31_October_2010&action=edit 31]
|
|
|