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INSA-Lyon/12 July 2010
From 2010.igem.org
July 12
Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161
Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis
Digestion:
| part | enzyme 1 | enzyme 2 |
|---|---|---|
| BBa_B0015 | E | X |
| BBa_K115017 | E | X |
| BBa_K115017 | X | P |
| BBa_J23119 | E | S |
| BBa_E0040 | E | S |
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no gel picture available
Ligations + transformations: (during 3 hours at room temperature)
- BBa_J23119+BBa_K115017 in plasmid pSB1C3 (3A method): constitutive promoter + ARNth
- BBa_E0040+BBa_B0015: GFP + terminator
- BBa_J23119+BBa_K115017 : constitutive promoter + ARNth
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