Team:Bielefeld-Germany/Results/Sequencing

From 2010.igem.org

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===Own BioBricks===
===Own BioBricks===
-
The sequencing of the following of our own BioBricks was succesful and lead to the expected results:
 
-
* <partinfo>K389001</partinfo>
+
All our own BioBricks of major importance were sequenced via one-shot sequencing by IIT Biotech (Bielefeld, Germany). The results, sequence (FASTA) and trace files, were aligned to the BioBrick sequences stored in the Registry of Standard Biological Parts and further analyzed with the software tools ‘’Clone Manager Professional Suite 6.0’’ (Sci Ed Central) and ‘’FinchTV 1.4.0’’ (Geospiza, Inc.). Finally, sequences with high quality scores were uploaded under ‘’Physical DNA – BioBrick Blast’’. Apart from minor and negligible, e.g. single nucleotide differing base at the scar, all of our sequencing results completely match the sequences in the Registry of Standard Biological Parts. Therefore, we solely want to give an overview of our BioBrick sequencing status (completely sequenced or partially sequenced) on this page.
-
* <partinfo>K389002</partinfo>
 
-
* <partinfo>K389003</partinfo>
+
* <partinfo>K389001</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389001 Sequencing results here])
-
* <partinfo>K389004</partinfo>
+
* <partinfo>K389002</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389002 Sequencing results here])
-
* <partinfo>K389005</partinfo>
+
* <partinfo>K389003</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389003 Sequencing results here])
-
* <partinfo>K389010</partinfo> (not fully completed)
+
* <partinfo>K389004</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389004 Sequencing results here])
-
* <partinfo>K389011</partinfo> (not fully completed)
+
* <partinfo>K389005</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389005 Sequencing results here])
-
* <partinfo>K389012</partinfo> (not fully completed)
+
* <partinfo>K389010</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389010 Sequencing results here])
-
* <partinfo>K389013</partinfo> (not fully completed)
+
* <partinfo>K389011</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389011 Sequencing results here])
-
* <partinfo>K389015</partinfo> (not fully completed)
+
* <partinfo>K389012</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389012 Sequencing results here])
-
* <partinfo>K389016</partinfo> (not fully completed)
+
* <partinfo>K389013</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])
-
* <partinfo>K389050</partinfo>
+
* <partinfo>K389015</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])
 +
 
 +
* <partinfo>K389016</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389016 Sequencing results here])
 +
 
 +
* <partinfo>K389017</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389017 Sequencing results here])
 +
 
 +
* <partinfo>K389401</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389401 Sequencing results here])
 +
 
 +
* <partinfo>K389402</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389402 Sequencing results here])
 +
 
 +
* <partinfo>K389403</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389403 Sequencing results here])
 +
 
 +
* <partinfo>K389411</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389411 Sequencing results here])
 +
 
 +
* <partinfo>K389412</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389412 Sequencing results here])
 +
 
 +
* <partinfo>K389413</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])
 +
 
 +
 
 +
* <partinfo>K389421</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389421 Sequencing results here])
 +
 
 +
* <partinfo>K389422</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389422 Sequencing results here])
 +
 
 +
* <partinfo>K389423</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389423 Sequencing results here])
===Other BioBricks===
===Other BioBricks===
-
* <partinfo>K238008</partinfo> sequencing gave negative results - infos in registry are not correct!
+
* <partinfo>K238008</partinfo> Sequencing gave negative results - infos in registry are not correct! ([http://partsregistry.org/Part:BBa%20K238008:Experience More Info])
-
* <partinfo>K238011</partinfo> sequencing gave negative results - infos in registry are not correct!
+
* <partinfo>K238011</partinfo> Sequencing gave negative results - infos in registry are not correct! ([http://partsregistry.org/Part:BBa%20K238011:Experience More Info])

Revision as of 02:21, 28 October 2010

http://igem-bielefeld.de/img/banner_lab.png

Sequencing

Own BioBricks

All our own BioBricks of major importance were sequenced via one-shot sequencing by IIT Biotech (Bielefeld, Germany). The results, sequence (FASTA) and trace files, were aligned to the BioBrick sequences stored in the Registry of Standard Biological Parts and further analyzed with the software tools ‘’Clone Manager Professional Suite 6.0’’ (Sci Ed Central) and ‘’FinchTV 1.4.0’’ (Geospiza, Inc.). Finally, sequences with high quality scores were uploaded under ‘’Physical DNA – BioBrick Blast’’. Apart from minor and negligible, e.g. single nucleotide differing base at the scar, all of our sequencing results completely match the sequences in the Registry of Standard Biological Parts. Therefore, we solely want to give an overview of our BioBrick sequencing status (completely sequenced or partially sequenced) on this page.


  • <partinfo>K389001</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389001 Sequencing results here])
  • <partinfo>K389002</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389002 Sequencing results here])
  • <partinfo>K389003</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389003 Sequencing results here])
  • <partinfo>K389004</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389004 Sequencing results here])
  • <partinfo>K389005</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389005 Sequencing results here])
  • <partinfo>K389010</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389010 Sequencing results here])
  • <partinfo>K389011</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389011 Sequencing results here])
  • <partinfo>K389012</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389012 Sequencing results here])
  • <partinfo>K389013</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])
  • <partinfo>K389015</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])
  • <partinfo>K389016</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389016 Sequencing results here])
  • <partinfo>K389017</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389017 Sequencing results here])
  • <partinfo>K389401</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389401 Sequencing results here])
  • <partinfo>K389402</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389402 Sequencing results here])
  • <partinfo>K389403</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389403 Sequencing results here])
  • <partinfo>K389411</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389411 Sequencing results here])
  • <partinfo>K389412</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389412 Sequencing results here])
  • <partinfo>K389413</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389413 Sequencing results here])


  • <partinfo>K389421</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389421 Sequencing results here])
  • <partinfo>K389422</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389422 Sequencing results here])
  • <partinfo>K389423</partinfo> ([http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=BBa%20K389423 Sequencing results here])


Other BioBricks

  • <partinfo>K238008</partinfo> Sequencing gave negative results - infos in registry are not correct! ([http://partsregistry.org/Part:BBa%20K238008:Experience More Info])
  • <partinfo>K238011</partinfo> Sequencing gave negative results - infos in registry are not correct! ([http://partsregistry.org/Part:BBa%20K238011:Experience More Info])
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