Team:INSA-Lyon/Project/Stage3/Strategy/curli promoter

From 2010.igem.org

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<h3>Stage 3 : Strategy</h3>
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<h3>Design of Curli promoter</h3>
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<p> We choose to follow two differents strategies to obtain our systems of regulation. <br>
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<li> Thermoregulation</li><br>
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<p>For this system of regulation, we decided to synthesized directly the sequence of the <a href="https://2010.igem.org/wiki/index.php?title=Team:INSA-Lyon/Project/Stage3/Strategy/curli_promoter">curli promoter</a> with the igem restriction sites. In the same time a part with the gene ompR234 is created by PCR, from the genome of the chassi PHL818.
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Then we ligate an igem reporter gene with the curli promoter and we wanted to make a double transformation with ompR234 to see if our parts work as we hoped.
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The final plasmid construction of the thermometer RNA will contain a constitutive promoter(BBa_J23119), the thermometer RNA, the PPI-protein fused and a terminator.
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<li> Control under Arabinose</li><br>
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Revision as of 11:43, 26 October 2010




Design of Curli promoter



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