Team:INSA-Lyon/Project/Stage3/Strategy/construction1

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<h3> Construction for the regulation by arabinose </h3>
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<br><p>We can find below the details schemas of the three constructions requisite for this regulation
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<h2>Construction 1</h2>
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<p> Constitutive production of LuxR and LuxI proteins. Igem parts of luxR and luxI genes are digested and then ligated in the pSB1T3 tetracylcin resistant plasmid. This first construction is then digested by XbaI and PstI. In parallel, igem part of the constitutive promoter is digested by SpeI and PstI. Finally the products are ligated together. The construction obtained can produce constitutively LuxR and LuxI proteins in normal growth conditions (LB 37°C).</p><br /><br />
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<h3><font color="purple">Construction1 : Constitutive production of LuxR and LuxI proteins</font></h3><br><br>
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<p>Igem parts of luxR and luxI genes are digested and then ligated in the pSB1T3 tetracylcin resistant plasmid. This first construction is then digested by XbaI and PstI. In parallel, igem part of the constitutive promoter is digested by SpeI and PstI. Finally the products are ligated together. The construction obtained can produce constitutively LuxR and LuxI proteins in normal growth conditions (LB 37°C).</p><br /><br /></li>
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Revision as of 19:14, 27 October 2010




Construction for the regulation by arabinose


We can find below the details schemas of the three constructions requisite for this regulation

  1. LuxR and LuxI construction
  2. LuxR and HSL construction
  3. pBad and AraC construction


    Construction1 : Constitutive production of LuxR and LuxI proteins



    • Igem parts of luxR and luxI genes are digested and then ligated in the pSB1T3 tetracylcin resistant plasmid. This first construction is then digested by XbaI and PstI. In parallel, igem part of the constitutive promoter is digested by SpeI and PstI. Finally the products are ligated together. The construction obtained can produce constitutively LuxR and LuxI proteins in normal growth conditions (LB 37°C).



      • Figure 1 : Constitutive production of LuxR and LuxI proteins

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