Team:INSA-Lyon/Project/Stage3/Strategy

From 2010.igem.org

(Difference between revisions)

Revision as of 10:34, 26 October 2010

> Our Project

> Strategy

> Results

Stage 3 : Strategy

We choose to follow two differents strategies to obtain our systems of regulation.

Thermoregulation

For this system of regulation, we decided to synthesized directly the sequence of the curli promoter with the igem restriction sites. In the same time a part with the gene ompR234 is created by PCR, from the genome of the chassi PHL818. Then we ligate an igem reporter gene with the curli promoter and we wanted to make a double transformation with omp234 to see if our parts work as we hoped.

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 <h3>Stage 3 : Strategy</h3>
 <br>
-<p>First, we decided to pursue a computer-based approach using databases such that NCBI. We compared FAS I enzyme function for different organisms (mammals and bacteria). We therefore managed to show a high-level of similarity between the domains of the FAS I. </p>
+<p> We choose to follow two differents strategies to obtain our systems of regulation. <br>
-<br>
+<ol id="list_extra">
-<p>Second, we wanted to analyze these collected data to design a more evolved structure of the operon gene PHA. This is based on the hypothesis that a multi-functional enzyme would increase the yield of lipid production by E.coli.</p>
+<li> Thermoregulation</li><br>
+</ol>
+</p>
+<p>For this system of regulation, we decided to synthesized directly the sequence of the curli promoter with the igem restriction sites. In the same time a part with the gene ompR234 is created by PCR, from the genome of the chassi PHL818.
+Then we ligate an igem reporter gene with the curli promoter and we wanted to make a double transformation with omp234 to see if our parts work as we hoped.
+</p>
 <br>