Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/09
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=2010/10/09= | =2010/10/09= | ||
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
- | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
- | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
- | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
- | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
- | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
- | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
- | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
- | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
- | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
- | *2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-3] |
- | *2-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-7] |
- | *2-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
- | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
- | *6-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-3] |
- | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
- | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===2:Electrophoresis①=== | ===2:Electrophoresis①=== | ||
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<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
- | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
<Protocol><br /> | <Protocol><br /> | ||
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<Result><br /> | <Result><br /> | ||
- | [[Image:]] | + | [[Image:Matsuura_2010-10-09_18hr_03min.JPG]] |
+ | [[Image:Matsuura_2010-10-09_18hr_07min.JPG]] | ||
===4:Electrophoresis③=== | ===4:Electrophoresis③=== | ||
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<Result><br /> | <Result><br /> | ||
- | [[Image:]] | + | [[Image:Matsuura_2010-10-09_18hr_25min.JPG]] |
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===5:Electrophoresis④=== | ===5:Electrophoresis④=== | ||
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<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
- | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
- | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
- | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
- | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
- | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
- | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
- | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
- | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
- | *2-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
- | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
<Protocol><br /> | <Protocol><br /> | ||
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<Result><br /> | <Result><br /> | ||
- | [[Image:]] | + | [[Image:Matsuura_2010-10-09_23hr_03min.JPG]] |
+ | [[Image:Matsuura_2010-10-10_13hr_33min.JPG]] | ||
===6:DNA Digestion=== | ===6:DNA Digestion=== | ||
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<Sample, Materials><br /> | <Sample, Materials><br /> | ||
・PCR productions | ・PCR productions | ||
- | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
- | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
- | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
- | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
- | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
*vector | *vector | ||
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] |
Latest revision as of 02:58, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/09
1:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
2:Electrophoresis①
<Member>
Hitomi
<Sample>
PCR products
<Protocol>
See Protocol 8
3:Electrophoresis②
<Member>
Hitomi
<Sample>
PCR products (10/8)
<Protocol>
See Protocol 8
4:Electrophoresis③
<Member>
Hitomi
<Sample>
PCR products (10/9)
<Protocol>
See Protocol 8
5:Electrophoresis④
<Member>
Mariko
<Sample>
PCR products
<Protocol>
See Protocol 8
6:DNA Digestion
<Member>
Mariko
<Sample, Materials>
・PCR productions
<Protocol>
See Prptpcpl 9