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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/02
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
Transformation 9/28 | Transformation 9/28 | ||
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===2：DNA Ligation === | ===2：DNA Ligation === | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
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===3:Electrophoresis=== | ===3:Electrophoresis=== | ||
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<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> | ||
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[[Image:Matsuura_2010-10-02_20hr_46min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_20hr_46min.JPG]]<br /> | ||
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Latest revision as of 02:55, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/02
1:PCR
<Member>
mio,mizuki
<Sample>(temperature in annealing:℃)
Transformation 9/28
<Protocol>
See Protocol 2
2：DNA Ligation
＜Member＞
mizuki
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
3:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
