Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17

From 2010.igem.org

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=2010/09/17=
=2010/09/17=
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<Sample><br />(temperature in annealing)
<Sample><br />(temperature in annealing)
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*3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
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*6
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6]
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*8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===2：DNA Digestion===
===2：DNA Digestion===
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
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*1 (8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (8/31)
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*2 (9/6)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/6)
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*4 (8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (8/31)
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*5L (8/26)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L] (8/26)
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*7 (8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] (8/31)
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*8 (8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (8/31)
・Digest enzyme
・Digest enzyme
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===3:Electrophoresis①===
===3:Electrophoresis①===
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<Sample><br />
<Sample><br />
PCR products (9/17)
PCR products (9/17)
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*3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
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*6
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6]
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*8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
<Protocol><br />
<Protocol><br />
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<Sample><br />
<Sample><br />
Digestion products (9/17)
Digestion products (9/17)
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*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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*7
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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*8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
<Protocol><br />
<Protocol><br />
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＜Sample＞<br />
＜Sample＞<br />
Digestion products(9/17)
Digestion products(9/17)
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*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*7
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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*8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
(After electrophoresis)
(After electrophoresis)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===6：DNA Ligation ===
===6：DNA Ligation ===
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＜Sample＞<br />
＜Sample＞<br />
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*7(9/17)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](9/17)   
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*8(9/17)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8](9/17)
(after digestion)
(after digestion)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]

Latest revision as of 18:16, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/09/17

1:PCR

<Member>
mio

<Sample>
(temperature in annealing)

<Protocol>
See Protocol 1

2：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/6)
  • 4 (8/31)
  • 5L (8/26)
  • 7 (8/31)
  • 8 (8/31)

・Digest enzyme

  • 1,4,8 : AvrⅡ
  • 2,7 : NheⅠ
  • 5L : SpeⅠ


＜Protocol＞
See Prptpcpl 9

3:Electrophoresis①

<Member>
mio

<Sample>
PCR products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 17hr 10min.JPG

4:Electrophoresis②

<Member>
mizuki

<Sample>
Digestion products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 18hr 05min.JPG

5：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products(9/17)

(After electrophoresis)

＜Protocol＞
See Protocol 4

6：DNA Ligation

＜Member＞
mizuki

＜Sample＞

  • 7(9/17)
  • 8(9/17)

(after digestion)

＜Protocol＞
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/17"