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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/23
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(New page: ===1：DNA Digestion=== ＜Member＞<br /> Hitomi ＜Sample, Materials＞<br /> ・PCR productions *1 *2 *4 *5 ＜Protocol＞<br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Proj...) |
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| + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
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| + | =2010/09/23= | ||
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===1：DNA Digestion=== | ===1：DNA Digestion=== | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
| Line 5: | Line 9: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
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===2:PCR=== | ===2:PCR=== | ||
| Line 18: | Line 21: | ||
Hitomi | Hitomi | ||
| - | <Sample | + | <Sample>(temperature in annealing)<br /> |
| - | *7(67.5) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](67.5) |
| - | *9(57,61,69) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9](57,61,69) |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===3：DNA extraction=== | ===3：DNA extraction=== | ||
| Line 44: | Line 45: | ||
<Sample><br /> | <Sample><br /> | ||
Digestion products. | Digestion products. | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 54: | Line 55: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-09-23_20hr_35min.JPG]] |
===5:Electrophoresis=== | ===5:Electrophoresis=== | ||
| Line 65: | Line 66: | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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===6：DNA extraction from gels=== | ===6：DNA extraction from gels=== | ||
| Line 87: | Line 84: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
| - | *2 (9/23) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/23) |
| - | *4 (9/23) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/23) |
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
Latest revision as of 18:17, 24 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/09/23
1：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
・PCR productions
＜Protocol＞
See Prptpcpl 9
2:PCR
<Member>
Hitomi
<Sample>(temperature in annealing)
<Protocol>
See Protocol 1
3：DNA extraction
＜Member＞
Hitomi
＜Sample＞
PCR products(9/23)
＜Protocol＞
See Protocol 4
4:Electrophoresis
<Member>
Hitomi
<Sample>
Digestion products.
<Protocol>
See Protocol 8
<Result>
5:Electrophoresis
<Member>
Hitomi
<Sample>
PCR products (9/23)
<Protocol>
See Protocol 8
6：DNA extraction from gels
＜Member＞
Mariko,Hitomi
＜Sample＞
Digestion products (9/23)
(After electrophoresis)
＜Protocol＞
See Protocol 4
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
＜Protocol＞
See Prptpcpl 9
