Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17

From 2010.igem.org

(Difference between revisions)
(New page: ===1:PCR=== <Member><br /> mio <Sample><br />(temperature in annealing) *3 *6 *8 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Ph...)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/17=
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===1:PCR===
===1:PCR===
<Member><br />
<Member><br />
mio
mio
-
 
<Sample><br />(temperature in annealing)
<Sample><br />(temperature in annealing)
-
*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
-
*6
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6]
-
*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
-
 
-
 
-
 
===2：DNA Digestion===
===2：DNA Digestion===
＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
-
 
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
-
*1 (8/31)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (8/31)
-
*2 (9/6)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/6)
-
*4 (8/31)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (8/31)
-
*5L (8/26)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L] (8/26)
-
*7 (8/31)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] (8/31)
-
*8 (8/31)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (8/31)
・Digest enzyme
・Digest enzyme
Line 38: Line 36:
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
-
 
-
 
===3:Electrophoresis①===
===3:Electrophoresis①===
<Member><br />
<Member><br />
mio
mio
-
 
<Sample><br />
<Sample><br />
PCR products (9/17)
PCR products (9/17)
-
*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
-
*6
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6]
-
*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
 
+
[[Image:Matsuura_2010-09-17_17hr_10min.JPG]]
-
 
+
===4:Electrophoresis②===
===4:Electrophoresis②===
<Member><br />
<Member><br />
mizuki
mizuki
-
 
<Sample><br />
<Sample><br />
Digestion products (9/17)
Digestion products (9/17)
-
*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
-
*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
-
*5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
-
*7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
-
*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
 
+
[[Image:Matsuura_2010-09-17_18hr_05min.JPG]]
-
 
+
===5：DNA extraction from gels===
===5：DNA extraction from gels===
＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
-
 
＜Sample＞<br />
＜Sample＞<br />
Digestion products(9/17)
Digestion products(9/17)
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
-
*7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
-
*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
(After electrophoresis)
(After electrophoresis)
-
 
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
 
-
 
===6：DNA Ligation ===
===6：DNA Ligation ===
＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
-
 
＜Sample＞<br />
＜Sample＞<br />
-
*7(9/17)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7](9/17)   
-
*8(9/17)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8](9/17)
(after digestion)
(after digestion)
-
 
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]

Latest revision as of 18:16, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/09/17

1:PCR

<Member>
mio

<Sample>
(temperature in annealing)

<Protocol>
See Protocol 1

2：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/6)
  • 4 (8/31)
  • 5L (8/26)
  • 7 (8/31)
  • 8 (8/31)

・Digest enzyme

  • 1,4,8 : AvrⅡ
  • 2,7 : NheⅠ
  • 5L : SpeⅠ


＜Protocol＞
See Prptpcpl 9

3:Electrophoresis①

<Member>
mio

<Sample>
PCR products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 17hr 10min.JPG

4:Electrophoresis②

<Member>
mizuki

<Sample>
Digestion products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 18hr 05min.JPG

5：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products(9/17)

(After electrophoresis)

＜Protocol＞
See Protocol 4

6：DNA Ligation

＜Member＞
mizuki

＜Sample＞

  • 7(9/17)
  • 8(9/17)

(after digestion)

＜Protocol＞
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/17"