"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/26
From 2010.igem.org
(Difference between revisions)
(→1：PCR) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
| + | |||
=2010/08/26= | =2010/08/26= | ||
| Line 99: | Line 100: | ||
＜Result＞<br /> | ＜Result＞<br /> | ||
| - | [[Image:Matsuura_2010-08- | + | [[Image:Matsuura_2010-08-06_20hr_02min_(5).JPG]] |
==5：Transformation with ECOS competent E-coli(NIPPON GENE)== | ==5：Transformation with ECOS competent E-coli(NIPPON GENE)== | ||
Latest revision as of 18:10, 24 October 2010
E.coli Pattern Formation Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/08/26
1：PCR
＜Member＞
Mariko, mizuki
＜Sample＞
・E-coli (having BBa_K208017 plasmid)
・E-coli (having BBa_I732901 plasmid)
・E-coli(JM109)
＜Protocol＞
See Protocol 2
・Tube(higher temperature/lower temperature in annealing)
2. JM109 (71.5℃/70.0)
4. Promoter(71.5/70.0)
5. RBS~signal (71.5/70.0)
6. lacZ (66.0/63.0)
Total 8 tubes.
2：DNA extraction
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 4
3：DNA concentration measurement
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
| concentration | A320 | A260/A280 | A260/A230 | |
|---|---|---|---|---|
| 2H | 95.5 | 14.84 | 2.011 | 0.054 |
| 2L | 43.0 | -0.025 | 1.792 | 0.203 |
| 4H | 75.0 | 0.044 | 1.852 | 0.346 |
| 4L | 63.0 | -0.004 | 1.881 | 0.565 |
| 5H | 35.0 | 2.96 | 2.000 | 0.160 |
| 5L | 31.0 | -0.020 | 1.873 | 0.071 |
| 6H | 54.5 | -0.078 | 1.946 | 0.215 |
| 6L | 72.0 | 0.501 | 2.257 | 0.029 |
4：Electrophoresis
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
5：Transformation with ECOS competent E-coli(NIPPON GENE)
＜Member＞
nito
＜Sample＞
・ECOS competent E-coli JM109(NIPPON GENE)
・Parts
- BBa_I13521
- BBa_B0010
- BBa_B0030
- BBa_J23110
- BBa_E0040
＜Protocol＞
SeeProtocol 7
