Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/26

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=2010/08/26=
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Total 8 tubes.
Total 8 tubes.
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==2：DNA extraction==
==2：DNA extraction==
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＜Result＞<br />
＜Result＞<br />
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[[Image:Matsuura_2010-08-26_16hr_14min.tif]]
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[[Image:Matsuura_2010-08-06_20hr_02min_(5).JPG]]
==5：Transformation with ECOS competent E-coli(NIPPON GENE)==
==5：Transformation with ECOS competent E-coli(NIPPON GENE)==

Latest revision as of 18:10, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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Contents

2010/08/26

1：PCR

＜Member＞
Mariko, mizuki


＜Sample＞
・E-coli (having BBa_K208017 plasmid)

・E-coli (having BBa_I732901 plasmid)

・E-coli(JM109)


＜Protocol＞
See Protocol 2

・Tube(higher temperature/lower temperature in annealing)
2. JM109 (71.5℃/70.0)
4. Promoter(71.5/70.0)
5. RBS~signal (71.5/70.0)
6. lacZ (66.0/63.0)

Total 8 tubes.

2：DNA extraction

＜Member＞
nito


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 4


3：DNA concentration measurement

＜Member＞
nito


＜Sample＞
・PCR products


＜Protocol＞

  1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
  2. Fall in drops 1 on the measuring machine.


＜Result＞

DNA concentration
concentration A320 A260/A280 A260/A230
2H 95.5 14.84 2.011 0.054
2L 43.0 -0.025 1.792 0.203
4H 75.0 0.044 1.852 0.346
4L 63.0 -0.004 1.881 0.565
5H 35.0 2.96 2.000 0.160
5L 31.0 -0.020 1.873 0.071
6H 54.5 -0.078 1.946 0.215
6L 72.0 0.501 2.257 0.029


4：Electrophoresis

＜Member＞
nito


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (5).JPG

5：Transformation with ECOS competent E-coli(NIPPON GENE)

＜Member＞
nito


＜Sample＞
・ECOS competent E-coli JM109(NIPPON GENE)

・Parts

  • BBa_I13521
  • BBa_B0010
  • BBa_B0030
  • BBa_J23110
  • BBa_E0040


＜Protocol＞
SeeProtocol 7

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/08/26"