Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/06

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/08/06=
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==1：Making LB plate (20ml×20)==
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＜Member＞<br />
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Mariko, tomy, easily, naoto, nito, Taka, watachin
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==Experiment1：Making LB plate (20ml×20)==
 
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＜Member＞Mariko, tomy, easily, naoto, nito, Taka, watachin
 
＜Materials＞<br />
＜Materials＞<br />
・Yeast extract 2.0g<br />
・Yeast extract 2.0g<br />
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＜Protocol＞
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＜Protocol＞<br />
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1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.
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4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.
4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.
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==2：Scattering E-coli (having BioBrick plasmid) on LB plate==
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＜Member＞<br />
 +
tomy, easily, naoto, nito
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+
＜Materials＞<br />
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==Experiment2；Scattering E-coli (having BioBrick plasmid) on LB plate==
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＜Member＞tomy, easily, naoto, nito
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＜Materials＞
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・LB plate×4
・LB plate×4
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・E-coli (having BBa_K208017 plasmid)
・E-coli (having BBa_K208017 plasmid)
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<Protocol＞
 
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1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.
 
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2 Incubated these plate at 37℃.
 
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==Experiment2；Scattering E-coli (having BioBrick plasmid) on LB plate==
 
-
＜Member＞tomy, easily, naoto, nito
 
-
＜Materials＞<br />
 
-
・LB plate×4<br />
 
-
 
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・E-coli (having BBa_I1732901 plasmid)<br />
 
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・E-coli (having BBa_K208017 plasmid)<br />
 
<Protocol＞<br />
<Protocol＞<br />
-
 
1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.
1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.
2 Incubated these plate at 37℃.
2 Incubated these plate at 37℃.

Latest revision as of 18:08, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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2010/08/06

1：Making LB plate (20ml×20)

＜Member＞
Mariko, tomy, easily, naoto, nito, Taka, watachin


＜Materials＞
・Yeast extract 2.0g

・Peptone 2.0g

・Glucose 40.0g

・NaCl 2.0g

・Agerose 6.0g

・Ampcillim 0.4mg

・Dropped water 400ml


＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.

2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)

3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.

4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.

2：Scattering E-coli (having BioBrick plasmid) on LB plate

＜Member＞
tomy, easily, naoto, nito


＜Materials＞
・LB plate×4

・E-coli (having BBa_I1732901 plasmid)

・E-coli (having BBa_K208017 plasmid)


<Protocol＞
1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.

2 Incubated these plate at 37℃.

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