"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
(Difference between revisions)
(→10:Electrophoresis) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
| + | |||
=2010/10/14= | =2010/10/14= | ||
| - | |||
===1:Electrophoresis=== | ===1:Electrophoresis=== | ||
| Line 12: | Line 13: | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]] | [[Image:Matsuura_2010-10-14_14hr_29min.JPG]] | ||
| + | |||
===2：DNA extraction=== | ===2：DNA extraction=== | ||
| Line 32: | Line 32: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
| + | |||
===3：DNA Ligation === | ===3：DNA Ligation === | ||
| Line 47: | Line 48: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
| + | |||
===4：DNA Digestion=== | ===4：DNA Digestion=== | ||
| Line 62: | Line 64: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
| + | |||
===5:PCR=== | ===5:PCR=== | ||
| Line 74: | Line 77: | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9] | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| + | |||
===6：DNA extraction from gels=== | ===6：DNA extraction from gels=== | ||
| Line 85: | Line 88: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | ||
| - | |||
(After electrophoresis) | (After electrophoresis) | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
| + | |||
===7：DNA Digestion=== | ===7：DNA Digestion=== | ||
| Line 110: | Line 112: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
| + | |||
===8:PCR=== | ===8:PCR=== | ||
| Line 121: | Line 124: | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| + | |||
===9：DNA extraction from gels=== | ===9：DNA extraction from gels=== | ||
| Line 128: | Line 132: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
*9-7 | *9-7 | ||
| - | |||
(After electrophoresis) | (After electrophoresis) | ||
| Line 134: | Line 137: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
| + | |||
===10:Electrophoresis=== | ===10:Electrophoresis=== | ||
| Line 149: | Line 153: | ||
<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-14_22hr_56min.JPG]] | [[Image:Matsuura_2010-10-14_22hr_56min.JPG]] | ||
| + | |||
===11：DNA extraction from gels=== | ===11：DNA extraction from gels=== | ||
| Line 155: | Line 160: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
(After electrophoresis) | (After electrophoresis) | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
Latest revision as of 02:59, 25 October 2010
E.coli Pattern Formation Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
2：DNA extraction
＜Member＞
nito
＜Sample＞
＜Protocol＞
See Protocol 4
3：DNA Ligation
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
4：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
6：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
8:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
9：DNA extraction from gels
＜Member＞
nito
＜Sample＞
- 9-7
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
10:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
11：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
