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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
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=2010/10/14= | =2010/10/14= | ||
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===1:Electrophoresis=== | ===1:Electrophoresis=== | ||
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]] | [[Image:Matsuura_2010-10-14_14hr_29min.JPG]] | ||
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===2：DNA extraction=== | ===2：DNA extraction=== | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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===3：DNA Ligation === | ===3：DNA Ligation === | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
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===4：DNA Digestion=== | ===4：DNA Digestion=== | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
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===5:PCR=== | ===5:PCR=== | ||
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9] | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===6：DNA extraction from gels=== | ===6：DNA extraction from gels=== | ||
| Line 85: | Line 88: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | ||
| - | |||
(After electrophoresis) | (After electrophoresis) | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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===7：DNA Digestion=== | ===7：DNA Digestion=== | ||
| Line 110: | Line 112: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
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===8:PCR=== | ===8:PCR=== | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
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===9：DNA extraction from gels=== | ===9：DNA extraction from gels=== | ||
| Line 129: | Line 132: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
*9-7 | *9-7 | ||
| - | |||
(After electrophoresis) | (After electrophoresis) | ||
| Line 135: | Line 137: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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===10:Electrophoresis=== | ===10:Electrophoresis=== | ||
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<Sample><br /> | <Sample><br /> | ||
*vector | *vector | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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<Result><br /> | <Result><br /> | ||
| Line 159: | Line 160: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
(After electrophoresis) | (After electrophoresis) | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
Latest revision as of 02:59, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
2：DNA extraction
＜Member＞
nito
＜Sample＞
＜Protocol＞
See Protocol 4
3：DNA Ligation
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
4：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
6：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
8:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
9：DNA extraction from gels
＜Member＞
nito
＜Sample＞
- 9-7
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
10:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
11：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
