Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

(Difference between revisions)
(2:DNA extraction)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
=2010/10/14=
=2010/10/14=
-
 
===1:Electrophoresis===
===1:Electrophoresis===
Line 12: Line 13:
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
 +
===2:DNA extraction===
===2:DNA extraction===
Line 32: Line 32:
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===3:DNA Ligation ===
===3:DNA Ligation ===
Line 38: Line 39:
<Sample><br />
<Sample><br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*1-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*10-2-3  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-3]
-
*10-2-9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
-
*7-8-10  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
(after digestion)
(after digestion)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
 +
===4:DNA Digestion===
===4:DNA Digestion===
Line 62: Line 64:
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
===5:PCR===
===5:PCR===
Line 69: Line 72:
<Sample><br />
<Sample><br />
*vector
*vector
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*1-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*10-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-3]
-
*7-8-10
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
-
*10-2-9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
 +
===6:DNA extraction from gels===
===6:DNA extraction from gels===
Line 84: Line 87:
<Sample><br />
<Sample><br />
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
(After electrophoresis)
(After electrophoresis)
-
 
<Protocol><br />
<Protocol><br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===7:DNA Digestion===
===7:DNA Digestion===
Line 98: Line 100:
<Sample, Materials><br />
<Sample, Materials><br />
・PCR productions
・PCR productions
-
*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*1-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
-
*10-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
・Digest enzyme
・Digest enzyme
Line 110: Line 112:
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
===8:PCR===
===8:PCR===
Line 116: Line 119:
<Sample><br />
<Sample><br />
-
*9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
 +
===9:DNA extraction from gels===
===9:DNA extraction from gels===
Line 129: Line 132:
<Sample><br />
<Sample><br />
*9-7
*9-7
-
 
(After electrophoresis)
(After electrophoresis)
Line 135: Line 137:
<Protocol><br />
<Protocol><br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===10:Electrophoresis===
===10:Electrophoresis===
Line 142: Line 145:
<Sample><br />
<Sample><br />
*vector
*vector
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
Line 159: Line 160:
<Sample><br />
<Sample><br />
-
*9-7   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]  
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
 
+
(After electrophoresis)
(After electrophoresis)
-
 
<Protocol><br />
<Protocol><br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 14hr 29min.JPG


2:DNA extraction

<Member>
nito

<Sample>

<Protocol>
See Protocol 4


3:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10


4:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


5:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


6:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4


7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


8:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


9:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7

(After electrophoresis)


<Protocol>
SeeProtocol 4


10:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4