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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
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| + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
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=2010/10/15= | =2010/10/15= | ||
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===1：DNA extraction=== | ===1：DNA extraction=== | ||
| Line 7: | Line 8: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
*vector | *vector | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
| Line 21: | Line 21: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
| Line 35: | Line 34: | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
Mariko | Mariko | ||
| - | |||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
(After electrophoresis) | (After electrophoresis) | ||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
| Line 53: | Line 49: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
*vector | *vector | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
| + | |||
===5:Electrophoresis=== | ===5:Electrophoresis=== | ||
| Line 65: | Line 61: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
*vector | *vector | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-15_19hr_19min.JPG]] | [[Image:Matsuura_2010-10-15_19hr_19min.JPG]] | ||
| + | |||
===6:PCR=== | ===6:PCR=== | ||
| Line 83: | Line 78: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
| + | |||
===7：DNA Digestion=== | ===7：DNA Digestion=== | ||
| Line 94: | Line 90: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
*vector | *vector | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | ||
| + | |||
===8：DNA Ligation === | ===8：DNA Ligation === | ||
| Line 105: | Line 101: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
| + | |||
===9:PCR=== | ===9:PCR=== | ||
| Line 119: | Line 115: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
| + | |||
===10:PCR=== | ===10:PCR=== | ||
| Line 135: | Line 131: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
*vector | *vector | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
| Line 147: | Line 142: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
| Line 161: | Line 155: | ||
<Sample><br /> | <Sample><br /> | ||
| - | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
| - | *1-2-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7] |
| - | *10-2-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7] |
*JM109 | *JM109 | ||
| - | |||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | ||
| + | |||
===13:Electrophoresis=== | ===13:Electrophoresis=== | ||
| Line 175: | Line 169: | ||
<Sample><br /> | <Sample><br /> | ||
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] | |
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-16_03hr_24min.JPG]] |
===14:Electrophoresis=== | ===14:Electrophoresis=== | ||
<Member><br /> | <Member><br /> | ||
| - | + | nito | |
<Sample><br /> | <Sample><br /> | ||
| - | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] | |
| + | *vector | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
| - | [[Image:Matsuura_2010- | + | [[Image:Matsuura_2010-10-16_01hr_45min.JPG]] |
Latest revision as of 03:00, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
mio
<Sample>
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
- vector
- 9-7
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8
<Result>
