Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/15=
=2010/10/15=
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===1：DNA extraction===
===1：DNA extraction===
Line 7: Line 8:
＜Sample＞<br />
＜Sample＞<br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
*vector
*vector
-
 
＜Protocol＞<br />
＜Protocol＞<br />
Line 21: Line 21:
<Sample><br />
<Sample><br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
Line 35: Line 34:
＜Member＞<br />
＜Member＞<br />
Mariko
Mariko
-
 
＜Sample＞<br />
＜Sample＞<br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
 
+
(After electrophoresis)
(After electrophoresis)
-
 
＜Protocol＞<br />
＜Protocol＞<br />
Line 53: Line 49:
<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
*vector
*vector
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
 +
===5:Electrophoresis===
===5:Electrophoresis===
Line 65: Line 61:
<Sample><br />
<Sample><br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
*vector
*vector
-
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-15_19hr_19min.JPG]]
[[Image:Matsuura_2010-10-15_19hr_19min.JPG]]
 +
===6:PCR===
===6:PCR===
Line 83: Line 78:
<Sample><br />
<Sample><br />
-
*9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
 +
===7：DNA Digestion===
===7：DNA Digestion===
＜Member＞<br />
＜Member＞<br />
-
 
+
Hitomi
-
 
+
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
-
・PCR productions
+
*vector
-
*1L(8/25)  8μl
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*2L(8/26)  8μl
+
-
*4H(8/26)  16μl
+
-
*5H(8/26)  16μl
+
-
 
+
-
・Digest enzyme
+
-
*AvrⅡ
+
-
*NheⅠ
+
-
*SpeⅠ
+
-
 
+
＜Protocol＞<br />
＜Protocol＞<br />
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===8：DNA Ligation ===
===8：DNA Ligation ===
＜Member＞<br />
＜Member＞<br />
-
 
+
Hitomi
＜Sample＞<br />
＜Sample＞<br />
-
*1L(8/25) 
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*2L(8/26) 
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*4H(8/26) 
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7]
-
*5H(8/26) 
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
-
(after digestion)
+
-
 
+
＜Protocol＞<br />
＜Protocol＞<br />
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===9:PCR===
===9:PCR===
<Member><br />
<Member><br />
-
hito
+
mio
<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
-
*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
-
*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7]
-
*6
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*7
+
-
*8
+
-
*9
+
-
*10
+
-
*1-2
+
-
*4-5
+
-
*7-8-10
+
<Protocol><br />
<Protocol><br />
Line 152: Line 128:
===10:PCR===
===10:PCR===
<Member><br />
<Member><br />
-
hito
+
nito,mizuki
-
<Sample>(temperature in annealing:℃)<br />
+
<Sample><br />
-
*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
-
*2
+
*vector
-
*3
+
-
*4
+
-
*5
+
-
*6
+
-
*7
+
-
*8
+
-
*9
+
-
*10
+
-
*1-2
+
-
*4-5
+
-
*7-8-10
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
-
 
-
 
===11:Electrophoresis===
===11:Electrophoresis===
<Member><br />
<Member><br />
-
mio,mizuki
+
mio
<Sample><br />
<Sample><br />
-
PCR products (9/16)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
[[Image:Matsuura_2010-09-16_18hr_19min.JPG]]
+
[[Image:Matsuura_2010-10-15_22hr_32min.JPG]]
-
 
+
===12:Transformation===
===12:Transformation===
Line 194: Line 155:
<Sample><br />
<Sample><br />
-
*7-8-10
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
*JM109
*JM109
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
 +
===13:Electrophoresis===
===13:Electrophoresis===
<Member><br />
<Member><br />
-
mio,mizuki
+
nito
<Sample><br />
<Sample><br />
-
PCR products (9/16)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
[[Image:Matsuura_2010-09-16_18hr_19min.JPG]]
+
[[Image:Matsuura_2010-10-16_03hr_24min.JPG]]
-
 
+
===14:Electrophoresis===
===14:Electrophoresis===
<Member><br />
<Member><br />
-
mio,mizuki
+
nito
<Sample><br />
<Sample><br />
-
PCR products (9/16)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
 +
*vector
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
[[Image:Matsuura_2010-09-16_18hr_19min.JPG]]
+
[[Image:Matsuura_2010-10-16_01hr_45min.JPG]]

Latest revision as of 03:00, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/10/15

1：DNA extraction

＜Member＞
Hitomi

＜Sample＞

＜Protocol＞
SeeProtocol 4


2:Electrophoresis

<Member>
Hitomi

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 12hr 41min.JPG


3：DNA extraction from gels

＜Member＞
Mariko

＜Sample＞

(After electrophoresis)

＜Protocol＞
SeeProtocol 4


4:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


5:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 19hr 19min.JPG


6:PCR

<Member>
mio

<Sample>

<Protocol>
See Protocol 2


7：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞

＜Protocol＞
See Protocol 9


8：DNA Ligation

＜Member＞
Hitomi

＜Sample＞

＜Protocol＞
See Protocol 3


9:PCR

<Member>
mio

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


10:PCR

<Member>
nito,mizuki

<Sample>

  • 9
  • vector

<Protocol>
See Protocol 2

11:Electrophoresis

<Member>
mio

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 22hr 32min.JPG

12:Transformation

<Member>
nito

<Sample>

<Protocol>
See Protocol 7


13:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-16 03hr 24min.JPG

14:Electrophoresis

<Member>
nito

<Sample>

  • 9
  • vector

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-16 01hr 45min.JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/15"