Team:Bielefeld-Germany/Results/Used

From 2010.igem.org

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This is a list of all BioBricks we used from the registry or created by ourselves and a short explanation, why we used them. The list is sorted alphabetically.  
This is a list of all BioBricks we used from the registry or created by ourselves and a short explanation, why we used them. The list is sorted alphabetically.  
-
* <partinfo>B0017</partinfo>
+
{|style="border-collapse: collapse; border-width: 1px; border-style: solid; border-color: #000"
 +
|-
 +
!style="border-style: solid; border-width: 1px"| BioBrick (name and partnumber)
 +
!style="border-style: solid; border-width: 1px"| Status
 +
!style="border-style: solid; border-width: 1px"| Comment
 +
|-
 +
|style="border-style: solid; border-width: 1px"| double terminator, <partinfo>B0017</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* this contains two <partinfo>B0010</partinfo> terminators
 +
* we used this because of problems mentioned in registry with <partinfo>B0012</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| RBS, <partinfo>B0034</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* we always assembled a strong RBS
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''lacI'' gene, <partinfo>C0012</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* used this to build the tightly regulated ''lac''-operon <partinfo>K389050</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| mRFP, <partinfo>E1010</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* this BioBrick was used as a reporter gene, ''e.g.'' in the construct <partinfo>K389013</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| lacIq promoter, <partinfo>I14032</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* used this to build the tightly regulated ''lac''-operon <partinfo>K389050</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| mRFP generator, <partinfo>J04450</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* this BioBrick was used as a visible selection marker for cloning other BioBricks into the <partinfo>pSB1C3</partinfo> plasmid
 +
|-
 +
|style="border-style: solid; border-width: 1px"| constitutive promoter, <partinfo>J23110</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* we always used a medium strong constitutive promoter from the registry to express our BioBricks
 +
* this BioBrick was used ''e.g.'' in <partinfo>K389010</partinfo> or <partinfo>K389011</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| R6K origin, <partinfo>J61001</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* this BioBrick was used to create a plasmid without ColE1 ori
 +
* the R6K ori only works in some ''E. coli'' strains, so it is used for a two plasmid screening system
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''virA'', <partinfo>K238008</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* we wanted to use the ''virA'' BioBrick from the registry but problems occurred, so we created our own ''virA'' from ''Agrobacterium tumefaciens'' C58 TI-plasmid
 +
* our own (working) ''virA'' is here: <partinfo>K389001</partinfo>
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''vir''-promoter, <partinfo>K238011</partinfo>
 +
|style="border-style: solid; border-width: 1px"| from registry
 +
|style="border-style: solid; border-width: 1px"|
 +
* same as the ''virA'' BioBrick - this one from the registry does not work properly, so we made a ''vir''-promoter by ourselves
 +
* the part that was sent to us is actually a ''tetR'' gene (<partinfo>C0040</partinfo>) under the control of a constitutive promoter (<partinfo>J23105</partinfo>) before a double terminator (<partinfo>B0015</partinfo>, compare our sequencing results)
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''virA'', <partinfo>K389001</partinfo>
 +
|style="border-style: solid; border-width: 1px"| new
 +
|style="border-style: solid; border-width: 1px"|
 +
* "our" ''virA''
 +
* illegal restriction site removed by site-directed mutagenesis
 +
* isolated from ''A. tumefaciens'' C58 TI-plasmid
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''virG'', <partinfo>K389002</partinfo>
 +
|style="border-style: solid; border-width: 1px"| synthesized
 +
|style="border-style: solid; border-width: 1px"|
 +
* ''virG'' that works in ''E. coli'' without ''rpoA'' gene from ''A. tumefaciens''
 +
* ...
 +
|-
 +
|style="border-style: solid; border-width: 1px"| ''vir''-promoter, <partinfo>K389003</partinfo>
 +
|style="border-style: solid; border-width: 1px"| new
 +
|style="border-style: solid; border-width: 1px"|
 +
* "our" ''vir''-promoter
 +
* isolated from ''A. tumefaciens'' C58 TI-plasmid
 +
|-
 +
|style="border-style: solid; border-width: 1px"| firefly luciferase, <partinfo>K389004</partinfo>
 +
|style="border-style: solid; border-width: 1px"| new
 +
|style="border-style: solid; border-width: 1px"|
 +
* "our" luciferase
 +
* sensitive reporter gene
 +
* isolated from Promega's pGL4.10luc2 plasmid
 +
|-
 +
|style="border-style: solid; border-width: 1px"| Kanamycin resistance gene, <partinfo>K389005</partinfo>
 +
|style="border-style: solid; border-width: 1px"| new
 +
|style="border-style: solid; border-width: 1px"|
 +
* "our" antibiotic resistance
 +
* used for directed evolution and mutated ''virA'' screenings, respectively
 +
* isolated from the BioBrick <partinfo>P1003</partinfo>
 +
|}
-
* <partinfo>B0034</partinfo>
 
-
 
-
* <partinfo>C0012</partinfo>
 
-
 
-
* <partinfo>E1010</partinfo>
 
-
 
-
* <partinfo>I14032</partinfo>
 
-
 
-
* <partinfo>J04450</partinfo>
 
-
 
-
* <partinfo>J23110</partinfo>
 
-
 
-
* <partinfo>J61001</partinfo>
 
-
 
-
* <partinfo>K238008</partinfo>
 
-
 
-
* <partinfo>K238011</partinfo>
 
-
 
-
* <partinfo>K389001</partinfo>
 
-
 
-
* <partinfo>K389002</partinfo>
 
-
 
-
* <partinfo>K389003</partinfo>
 
-
 
-
* <partinfo>K389004</partinfo>
 
-
 
-
* <partinfo>K389005</partinfo>
 
* <partinfo>K389010</partinfo>
* <partinfo>K389010</partinfo>

Revision as of 13:39, 28 September 2010

{{{1}}}

Used BioBricks

This is a list of all BioBricks we used from the registry or created by ourselves and a short explanation, why we used them. The list is sorted alphabetically.

BioBrick (name and partnumber) Status Comment
double terminator, <partinfo>B0017</partinfo> from registry
  • this contains two <partinfo>B0010</partinfo> terminators
  • we used this because of problems mentioned in registry with <partinfo>B0012</partinfo>
RBS, <partinfo>B0034</partinfo> from registry
  • we always assembled a strong RBS
lacI gene, <partinfo>C0012</partinfo> from registry
  • used this to build the tightly regulated lac-operon <partinfo>K389050</partinfo>
mRFP, <partinfo>E1010</partinfo> from registry
  • this BioBrick was used as a reporter gene, e.g. in the construct <partinfo>K389013</partinfo>
lacIq promoter, <partinfo>I14032</partinfo> from registry
  • used this to build the tightly regulated lac-operon <partinfo>K389050</partinfo>
mRFP generator, <partinfo>J04450</partinfo> from registry
  • this BioBrick was used as a visible selection marker for cloning other BioBricks into the <partinfo>pSB1C3</partinfo> plasmid
constitutive promoter, <partinfo>J23110</partinfo> from registry
  • we always used a medium strong constitutive promoter from the registry to express our BioBricks
  • this BioBrick was used e.g. in <partinfo>K389010</partinfo> or <partinfo>K389011</partinfo>
R6K origin, <partinfo>J61001</partinfo> from registry
  • this BioBrick was used to create a plasmid without ColE1 ori
  • the R6K ori only works in some E. coli strains, so it is used for a two plasmid screening system
virA, <partinfo>K238008</partinfo> from registry
  • we wanted to use the virA BioBrick from the registry but problems occurred, so we created our own virA from Agrobacterium tumefaciens C58 TI-plasmid
  • our own (working) virA is here: <partinfo>K389001</partinfo>
vir-promoter, <partinfo>K238011</partinfo> from registry
  • same as the virA BioBrick - this one from the registry does not work properly, so we made a vir-promoter by ourselves
  • the part that was sent to us is actually a tetR gene (<partinfo>C0040</partinfo>) under the control of a constitutive promoter (<partinfo>J23105</partinfo>) before a double terminator (<partinfo>B0015</partinfo>, compare our sequencing results)
virA, <partinfo>K389001</partinfo> new
  • "our" virA
  • illegal restriction site removed by site-directed mutagenesis
  • isolated from A. tumefaciens C58 TI-plasmid
virG, <partinfo>K389002</partinfo> synthesized
  • virG that works in E. coli without rpoA gene from A. tumefaciens
  • ...
vir-promoter, <partinfo>K389003</partinfo> new
  • "our" vir-promoter
  • isolated from A. tumefaciens C58 TI-plasmid
firefly luciferase, <partinfo>K389004</partinfo> new
  • "our" luciferase
  • sensitive reporter gene
  • isolated from Promega's pGL4.10luc2 plasmid
Kanamycin resistance gene, <partinfo>K389005</partinfo> new
  • "our" antibiotic resistance
  • used for directed evolution and mutated virA screenings, respectively
  • isolated from the BioBrick <partinfo>P1003</partinfo>


  • <partinfo>K389010</partinfo>
  • <partinfo>K389011</partinfo>
  • <partinfo>K389012</partinfo>
  • <partinfo>K389013</partinfo>
  • <partinfo>K389014</partinfo>
  • <partinfo>K389015</partinfo>
  • <partinfo>K389016</partinfo>
  • <partinfo>K389017</partinfo>
  • <partinfo>K389050</partinfo>
  • <partinfo>P1003</partinfo>
  • <partinfo>P1010</partinfo>
  • <partinfo>pSB1AT3</partinfo>
  • <partinfo>pSB1C3</partinfo>
  • <partinfo>R0010</partinfo>
Retrieved from "http://2010.igem.org/Team:Bielefeld-Germany/Results/Used"