Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/12=
=2010/10/12=
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===1：DNA extraction===
===1：DNA extraction===
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＜Sample＞<br />
＜Sample＞<br />
-
*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*10-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===2：DNA Digestion===
===2：DNA Digestion===
Line 21: Line 22:
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR product
・PCR product
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*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
 
+
-
・Digest enzyme
+
-
*Avr2
+
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===3:PCR===
===3:PCR===
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<Sample><br />
<Sample><br />
-
*9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
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*1-2-3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*4-5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
 +
===4:Plasmid extraction from E-coli===
===4:Plasmid extraction from E-coli===
<Member><br />
<Member><br />
-
taka
+
Takahiro
<Sample><br />
<Sample><br />
-
*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*1-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-3]
-
*1-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-5]
-
*1-6
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-6]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
 +
===5：DNA Ligation ===
===5：DNA Ligation ===
＜Member＞<br />
＜Member＞<br />
-
mio,mizuki
+
nito
＜Sample＞<br />
＜Sample＞<br />
-
*1-2   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]  
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
-
*6A-7
+
-
*7-8-10
+
-
(after digestion)
+
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
-
==3：DNA Digestion==
+
 
 +
===6：DNA Digestion===
＜Member＞<br />
＜Member＞<br />
-
 
+
nito
-
 
+
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
-
*1L(8/25)  8μl
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H]
-
*2L(8/26)  8μl
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*4H(8/26)  16μl
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
-
*5H(8/26)  16μl
+
-
 
+
-
・Digest enzyme
+
-
*AvrⅡ
+
-
*NheⅠ
+
-
*SpeⅠ
+
-
 
+
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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Contents

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

＜Protocol＞
See Protocol 4


2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

＜Protocol＞
See Prptpcpl 9


3:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 2


4:Plasmid extraction from E-coli

<Member>
Takahiro

<Sample>

<Protocol>
See Protocol 6


5：DNA Ligation

＜Member＞
nito

＜Sample＞

＜Protocol＞
See Protocol 10


6：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

＜Protocol＞
See Protocol 9

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/12"