Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/12=
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===1：DNA extraction===
===1：DNA extraction===
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＜Sample＞<br />
＜Sample＞<br />
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*1-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*10-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===7：DNA Digestion===
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===2：DNA Digestion===
＜Member＞<br />
＜Member＞<br />
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nito
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Mariko
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
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・PCR productions
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・PCR product
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*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*2
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-
*3
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-
*4
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-
*6
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-
*10
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-
 
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・Digest enzyme
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-
*Avr2
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*Nhe1
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===1:PCR===
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===3:PCR===
<Member><br />
<Member><br />
-
hito
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nito
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<Sample>(temperature in annealing:℃)<br />
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<Sample><br />
-
*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
-
*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
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*3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
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*4
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*5
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*6
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*7
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*8
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*9
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*10
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*1-2
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*4-5
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*7-8-10
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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===4:Plasmid extraction from E-coli===
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<Member><br />
 +
Takahiro
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 +
<Sample><br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-3]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-5]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-6]
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
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===5：DNA Ligation ===
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＜Member＞<br />
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nito
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＜Sample＞<br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] 
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
 +
 +
 +
===6：DNA Digestion===
 +
＜Member＞<br />
 +
nito
 +
 +
＜Sample, Materials＞<br />
 +
・PCR productions
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

＜Protocol＞
See Protocol 4


2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

＜Protocol＞
See Prptpcpl 9


3:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 2


4:Plasmid extraction from E-coli

<Member>
Takahiro

<Sample>

<Protocol>
See Protocol 6


5：DNA Ligation

＜Member＞
nito

＜Sample＞

＜Protocol＞
See Protocol 10


6：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

＜Protocol＞
See Protocol 9

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/12"