Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12
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(Difference between revisions)
(New page: =2010/10/12= ===1:DNA extraction=== <Member><br /> Mariko <Sample><br /> *1-2 *10-2 *2 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern...) |
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===1:DNA extraction=== | ===1:DNA extraction=== | ||
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<Sample><br /> | <Sample><br /> | ||
- | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
- | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
+ | |||
+ | |||
+ | ===2:DNA Digestion=== | ||
+ | <Member><br /> | ||
+ | Mariko | ||
+ | |||
+ | <Sample, Materials><br /> | ||
+ | ・PCR product | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
+ | |||
+ | |||
+ | ===3:PCR=== | ||
+ | <Member><br /> | ||
+ | nito | ||
+ | |||
+ | <Sample><br /> | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
+ | |||
+ | |||
+ | ===4:Plasmid extraction from E-coli=== | ||
+ | <Member><br /> | ||
+ | Takahiro | ||
+ | |||
+ | <Sample><br /> | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-3] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-5] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-6] | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ] | ||
+ | |||
+ | |||
+ | ===5:DNA Ligation === | ||
+ | <Member><br /> | ||
+ | nito | ||
+ | |||
+ | <Sample><br /> | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
+ | |||
+ | |||
+ | ===6:DNA Digestion=== | ||
+ | <Member><br /> | ||
+ | nito | ||
+ | |||
+ | <Sample, Materials><br /> | ||
+ | ・PCR productions | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] | ||
+ | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] | ||
+ | |||
+ | <Protocol><br /> | ||
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] |
Latest revision as of 02:59, 25 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/12
1:DNA extraction
<Member>
Mariko
<Sample>
<Protocol>
See Protocol 4
2:DNA Digestion
<Member>
Mariko
<Sample, Materials>
・PCR product
<Protocol>
See Prptpcpl 9
3:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 2
4:Plasmid extraction from E-coli
<Member>
Takahiro
<Sample>
<Protocol>
See Protocol 6
5:DNA Ligation
<Member>
nito
<Sample>
<Protocol>
See Protocol 10
6:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
<Protocol>
See Protocol 9