Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/11

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/11=
=2010/10/11=
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===Electrophoresis===
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===1:Electrophoresis===
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<Member>
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<Member><br />
nito
nito
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<Sample><br />
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<Sample>
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veutor+[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
veutor+[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
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<Protocol><br />
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<Protocol>
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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See Protocol8
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(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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<Result><br />
<Result><br />
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
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===PCR===
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===2:PCR===
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<Member>
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<Member><br />
nito
nito
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<Sample><br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
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<Sample>
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10+2]
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*9
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*1+2
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*10+2
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*2
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*vector
*vector
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<Protocol><br />
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<Protocol>
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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*Tube(temperature in annealing)<br />
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See Protocol2
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(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
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*Tube(temperature in annealing)
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:1.(60.0,50.0℃)
:1.(60.0,50.0℃)
:2.(55.0℃)
:2.(55.0℃)
Line 52: Line 36:
:4.(55.0℃)
:4.(55.0℃)
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===3:Electrophoresis===
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<Member><br />
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===Electrophoresis===
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<Member>
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nito
nito
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<Sample><br />
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<Sample>
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PCR products.
PCR products.
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:*9H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H]
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:*9L
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9L]
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:*1-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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:*10-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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:*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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:*vector
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*vector
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
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<Protocol>
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See Protocol8
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(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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<Result>
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[[Image:Matsuura_2010-10-12_00hr_16min.JPG]]
[[Image:Matsuura_2010-10-12_00hr_16min.JPG]]

Latest revision as of 02:58, 25 October 2010


E.coli Pattern Formation Project Notebook



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Contents

2010/10/11

1:Electrophoresis

<Member>
nito

<Sample>
veutor+7-8-10

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-11 01hr 51min.JPG

2:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1

  • Tube(temperature in annealing)
1.(60.0,50.0℃)
2.(55.0℃)
3.(55.0℃)
4.(55.0℃)

3:Electrophoresis

<Member>
nito

<Sample>
PCR products.

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-12 00hr 16min.JPG