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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/08
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(New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/10/= ===1:PCR①=== <Member><br /> Hitomi <Sample>(temperature in annealing:℃)<br /> *9 *12 *210 <Protocol><br /> See [https://2010.igem.org/T...) |
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| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
| - | =2010/10/= | + | |
| + | =2010/10/08= | ||
===1:PCR①=== | ===1:PCR①=== | ||
| Line 7: | Line 8: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===2:Electrophoresis=== | ===2:Electrophoresis=== | ||
| Line 21: | Line 21: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 29: | Line 29: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-08_16hr_46min.JPG]] |
| - | + | ||
===3：DNA Ligation=== | ===3：DNA Ligation=== | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
| - | |||
===4:PCR②=== | ===4:PCR②=== | ||
| Line 51: | Line 49: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===5：DNA extraction from gels=== | ===5：DNA extraction from gels=== | ||
| Line 64: | Line 61: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
Digestion products | Digestion products | ||
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
(After electrophoresis) | (After electrophoresis) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
Latest revision as of 02:57, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/08
1:PCR①
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
2:Electrophoresis
<Member>
nito
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
3：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
4:PCR②
<Member>
nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
5：DNA extraction from gels
＜Member＞
nito
＜Sample＞
Digestion products
(After electrophoresis)
＜Protocol＞
See Protocol 4
