Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/29

From 2010.igem.org

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=2010/09/29=
=2010/09/29=
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＜Sample＞<br />
＜Sample＞<br />
PCR products
PCR products
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*1 (9/27)①
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/27)①
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*1 (9/27)②
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/27)②
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*2 (9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/27)
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*3 (9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] (9/27)
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*4 (9/27)①
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/27)①
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*4 (9/27)②
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/27)②
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*5 (9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](9/27)
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*8 (9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (9/27)
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*10(9/27)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](9/27)
Digestion products (9/27)
Digestion products (9/27)
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*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
-
*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===2:Electrophoresis===
===2:Electrophoresis===
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<Sample><br />
<Sample><br />
PCR products (9/27)
PCR products (9/27)
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*1  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
-
*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
<Protocol><br />
<Protocol><br />
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＜Sample＞<br />
＜Sample＞<br />
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*1,2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1,2]
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*4,5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4,5]
(after digestion)
(after digestion)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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===4:PCR===
===4:PCR===
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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
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*5  (70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (70)
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*6  (63)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] (63)
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*7-8(70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8](70)
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*9  (58,63,69,70,72)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (58,63,69,70,72)
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*10 (58)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] (58)
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*1-2(72)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2](72)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]<br />
But today we used Kod.
But today we used Kod.

Latest revision as of 02:54, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
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19 20 21 22 23 24 25
26 27 28 29 30
October 2010
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10 11 12 13 14 15 16
17 18 19 20 21 22 23
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31

Contents

2010/09/29

1：DNA extraction

＜Member＞
nito

＜Sample＞
PCR products

  • 1 (9/27)①
  • 1 (9/27)②
  • 2 (9/27)
  • 3 (9/27)
  • 4 (9/27)①
  • 4 (9/27)②
  • 5(9/27)
  • 8 (9/27)
  • 10(9/27)

Digestion products (9/27)

＜Protocol＞
See Protocol 4

2:Electrophoresis

<Member>
nito

<Sample>
PCR products (9/27)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-29 14hr 34min.JPG

3：DNA Ligation

＜Member＞
nito

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 3

4:PCR

<Member>
mio,mizuki

<Sample>(temperature in annealing:℃)

  • 5 (70)
  • 6 (63)
  • 7-8(70)
  • 9 (58,63,69,70,72)
  • 10 (58)
  • 1-2(72)

<Protocol>
See Protocol 1
But today we used Kod.

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/29"