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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/25
From 2010.igem.org
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(New page: ===1：DNA extraction from gels=== ＜Member＞<br /> mizuki ＜Sample＞<br /> Digestion products *2 *4 *5 *8 (After electrophoresis) ＜Protocol＞<br /> See [https://2010.igem.org/Team...) |
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===1：DNA extraction from gels=== | ===1：DNA extraction from gels=== | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
| Line 5: | Line 9: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
Digestion products | Digestion products | ||
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
(After electrophoresis) | (After electrophoresis) | ||
| Line 20: | Line 24: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
(after digestion) | (after digestion) | ||
| Line 33: | Line 37: | ||
<Sample>(temperature in annealing)<br /> | <Sample>(temperature in annealing)<br /> | ||
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
<Protocol><br /> | <Protocol><br /> | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *7-8 (9/17) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] (9/17) |
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
| Line 60: | Line 64: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products (9/25) | PCR products (9/25) | ||
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
Ligation products (9/25) | Ligation products (9/25) | ||
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 72: | Line 76: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-09-25_17hr_28min.JPG]] |
| - | + | ||
===6：DNA extraction=== | ===6：DNA extraction=== | ||
| Line 91: | Line 94: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
| - | *7-8 (9/6) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] (9/6) |
| - | *10 (9/25) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] (9/25) |
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
Latest revision as of 02:53, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/09/25
1：DNA extraction from gels
＜Member＞
mizuki
＜Sample＞
Digestion products
(After electrophoresis)
＜Protocol＞
See Protocol 4
2：DNA Ligation
＜Member＞
mizuki
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 3
3:PCR
<Member>
mizuki
<Sample>(temperature in annealing)
<Protocol>
See Protocol 1
4：DNA extraction
＜Member＞
mizuki
＜Sample＞
- 7-8 (9/17)
＜Protocol＞
See Protocol 4
5:Electrophoresis
<Member>
mizuki
<Sample>
PCR products (9/25)
Ligation products (9/25)
<Protocol>
See Protocol 8
<Result>
6：DNA extraction
＜Member＞
mizuki
＜Sample＞
PCR products
＜Protocol＞
See Protocol 4
7：DNA Digestion
＜Member＞
mizuki
＜Sample, Materials＞
＜Protocol＞
See Prptpcpl 9
