Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/11

From 2010.igem.org

(Difference between revisions)
(New page: ===1:PCR=== <Member><br /> mio,easily <Sample><br /> *2 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPO...)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/11=
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===1:PCR===
===1:PCR===
<Member><br />
<Member><br />
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<Sample><br />
<Sample><br />
-
*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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(temperature in annealing)
(temperature in annealing)
*2:70.0℃
*2:70.0℃
-
 
-
 
===2:Digestion(1)===
===2:Digestion(1)===
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<Sample><br />
<Sample><br />
-
*1H:Avr2,FD Buffer
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1H]
-
*2(9/6):Nhe1、M Buffer
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2](9/6)
-
*4(8/30):Avr2,FD Buffer
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4](8/30)
-
*5(8/30):Spe1、M Buffer
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](8/30)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===3:Electrophoresis===
===3:Electrophoresis===
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<Result><br />
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[[Image:Matsuura_2010-08-06_20hr_02min_(20).JPG‎]]
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===4:DNA extraction===
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===4:Electrophoresis===
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<Member><br />
 +
mio
 +
 
 +
 
 +
<Sample><br />
 +
Digestion products (9/11)
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
 +
 
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
 +
 
 +
 
 +
<Result><br />
 +
[[Image:Matsuura_2010-08-06_20hr_02min_(22).JPG]]
 +
 
 +
===5:DNA extraction===
<Member><br />
<Member><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
 
+
===6:Digestion(2)===
-
 
+
-
===5:Digestion(2)===
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<Member><br />
<Member><br />
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<Sample><br />
<Sample><br />
-
*1(9/11)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1](9/11)
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
-
 
+
===7:Electrophoresis===
-
 
+
-
===6:Electrophoresis===
+
<Member><br />
<Member><br />
Mariko,mizuki
Mariko,mizuki
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Digestion products
Digestion products
-
*1(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1](9/11)
-
*4L(8/26)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4L](8/26)
-
*8H
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8H]
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<Result><br />
 +
[[Image:Matsuura_2010-09-11_20hr_25min.JPG]]
===8:DNA extraction===
===8:DNA extraction===
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<Sample><br />
<Sample><br />
-
*4(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4](9/11)
-
*5(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](9/11)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
 
-
 
===9:Ligation===
===9:Ligation===
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<Sample><br />
<Sample><br />
-
*4(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4](9/11)
-
*5(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](9/11)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
-
 
-
 
===10:DNA extraction===
===10:DNA extraction===
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<Sample><br />
<Sample><br />
-
*1(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1](9/11)
-
*2(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2](9/11)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
 
-
 
===12:Ligation===
===12:Ligation===
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<Sample><br />
<Sample><br />
-
*1(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1](9/11)
-
*2(9/11)
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2](9/11)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]

Latest revision as of 18:15, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/09/11

1:PCR

<Member>
mio,easily


<Sample>


<Protocol>
See Protocol 2

(temperature in annealing)

  • 2:70.0℃

2:Digestion(1)

<Member>
Mariko,mizuki


<Sample>

  • 1H
  • 2(9/6)
  • 4(8/30)
  • 5(8/30)


<Protocol>
See Prptpcpl 9

3:Electrophoresis

<Member>
Mariko,mio


<Sample>

  • PCR products(9/11)
  • Digestion products(9/11)


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (20).JPG

4:Electrophoresis

<Member>
mio


<Sample>
Digestion products (9/11)

<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (22).JPG

5:DNA extraction

<Member>
mizuki,easily


<Sample>
PCR products(9/11)


<Protocol>
See Protocol 4

6:Digestion(2)

<Member>
easily


<Sample>

  • 1(9/11)


<Protocol>
See Prptpcpl 9

7:Electrophoresis

<Member>
Mariko,mizuki


<Sample>
Digestion products


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-09-11 20hr 25min.JPG

8:DNA extraction

<Member>
mio,mizuki


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 4

9:Ligation

<Member>
Mariko,mio


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 10

10:DNA extraction

<Member>
Mariko,mio,mizuki


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 4

12:Ligation

<Member>
Mariko


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/11"