Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/08=
=2010/09/08=
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===PCR===
 
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<Member>
 
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===1:PCR===
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<Member><br />
Hitomi
Hitomi
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<Sample><brt />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2](ligation 2010/08/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5](ligation 2010/08/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7+8](ligation 2010/09/06)
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<Sample>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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*5
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*1+2(ligation 2010/08/31)
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*4+5(ligation 2010/08/31)
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*7+8(ligation 2010/09/06)
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<Protocol>
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See Protocol2
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(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
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*Tube(temperature in annealing)
*Tube(temperature in annealing)
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1.(71.0,70.5,70.0℃)<br />
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:1.(71.0,70.5,70.0℃)
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2.(72.0,71.5℃)<br />
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:2.(72.0,71.5℃)
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3.(70.0℃)<br />
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:3.(70.0℃)
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4.(70.0℃)
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:4.(70.0℃)
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:Total 12 tubes.
:Total 12 tubes.
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===Electrophoresis===
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===2:Electrophoresis===
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<Member><br />
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<Member>
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mizuki, Hitomi
mizuki, Hitomi
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<Sample><br />
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<Sample>
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PCR products.
PCR products.
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:*5H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H]
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:*5M
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5M]
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:*5L
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L]
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:*1-2H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2H]
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:*1-2L
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2L]
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:*4-5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
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:*7-8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8]
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<Protocol>
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See Protocol8
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(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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<Result>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
[[Image:Matsuura_2010-08-06_20hr_02min_(17).JPG]]
[[Image:Matsuura_2010-08-06_20hr_02min_(17).JPG]]

Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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2010/09/08

1:PCR

<Member>
Hitomi

<Sample><brt />

  • 5
  • 1+2(ligation 2010/08/31)
  • 4+5(ligation 2010/08/31)
  • 7+8(ligation 2010/09/06)

<Protocol>
See Protocol 2

  • Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)

Total 12 tubes.

2:Electrophoresis

<Member>
mizuki, Hitomi

<Sample>
PCR products.

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-08-06 20hr 02min (17).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/08"