"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/06
From 2010.igem.org
(New page: =2010/08/06= ==Experiment1：Making LB plate (20ml×20)== ＜Member＞Mariko, tomy, easily, naoto, nito, Taka, watachin ＜Materials＞<br /> ・Yeast extract 2.0g<br /> ・Peptone 2.0g...) |
|||
| (9 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
| + | |||
=2010/08/06= | =2010/08/06= | ||
| - | == | + | ==1：Making LB plate (20ml×20)== |
| - | + | ＜Member＞<br /> | |
| + | Mariko, tomy, easily, naoto, nito, Taka, watachin | ||
| + | |||
| + | |||
＜Materials＞<br /> | ＜Materials＞<br /> | ||
・Yeast extract 2.0g<br /> | ・Yeast extract 2.0g<br /> | ||
| Line 20: | Line 25: | ||
| - | ＜Protocol＞ | + | ＜Protocol＞<br /> |
| - | + | ||
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g. | 1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g. | ||
| Line 30: | Line 34: | ||
4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator. | 4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator. | ||
| + | ==2：Scattering E-coli (having BioBrick plasmid) on LB plate== | ||
| + | ＜Member＞<br /> | ||
| + | tomy, easily, naoto, nito | ||
| - | |||
| - | |||
＜Materials＞<br /> | ＜Materials＞<br /> | ||
| - | ・LB plate×4 | + | ・LB plate×4 |
| - | ・E-coli (having BBa_I1732901 plasmid) | + | ・E-coli (having BBa_I1732901 plasmid) |
| - | ・E-coli (having BBa_K208017 plasmid) | + | ・E-coli (having BBa_K208017 plasmid) |
<Protocol＞<br /> | <Protocol＞<br /> | ||
| - | |||
1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate. | 1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate. | ||
2 Incubated these plate at 37℃. | 2 Incubated these plate at 37℃. | ||
Latest revision as of 18:08, 24 October 2010
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/08/06
1：Making LB plate (20ml×20)
＜Member＞
Mariko, tomy, easily, naoto, nito, Taka, watachin
＜Materials＞
・Yeast extract 2.0g
・Peptone 2.0g
・Glucose 40.0g
・NaCl 2.0g
・Agerose 6.0g
・Ampcillim 0.4mg
・Dropped water 400ml
＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.
2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)
3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.
4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.
2：Scattering E-coli (having BioBrick plasmid) on LB plate
＜Member＞
tomy, easily, naoto, nito
＜Materials＞
・LB plate×4
・E-coli (having BBa_I1732901 plasmid)
・E-coli (having BBa_K208017 plasmid)
<Protocol＞
1 Sterilized Inoculation loop by flame. After Sterilization, pick up E-coli by Inoculation loop、and then scatter on LB plate.
2 Incubated these plate at 37℃.
