INSA-Lyon/15 September 2010

From 2010.igem.org





September 15th


Digestion :

  1. 8C/14K, 18A/24A (2 clones :one ligation of the 19/08, one of the 07/09) : with E+P
  2. 10L, 24A, I0500, 4O : with E

Verification of the digestion with an Agarose Gel electrophoris :

  1. 10L, 4O : they present the same bands on the wrong weight, maybe contamination
  2. I0500 : no DNA
  3. 24A : ok

Culture on plate of 4O/10L (ligation of the 13/09), 14K, I0500 and 5 new clones of phaA/phaB (ligation of 07/09)


Ligation of 1F/24A


First purification of phasin-intein


Measure of fluorescence and DO on the PHL1273 cultures

Start of 3 cultures of PHL1273 : waterbath 28°C and shaking speed at 120 rpm


PHL818 culture on GL-Tet (from 30/08/2010 petri plate culture stored at 4°C), incubation at 30°C overnight (then, stored at 4°C)








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