Team:Bielefeld-Germany/Results/Characterization

From 2010.igem.org

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|style="border-style: solid; border-width: 1px"| TOP10
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===<partinfo>K389011</partinfo>: VirA screening device===
===<partinfo>K389011</partinfo>: VirA screening device===

Revision as of 18:22, 9 October 2010

{{{1}}}

Contents

Tested BioBricks

We tested the following BioBricks:

<partinfo>K238008</partinfo>: virA

We made a restriction analysis and sequenced parts of this BioBrick. There were problems - more information within a short time.


<partinfo>BBa_K238011</partinfo>: vir-promoter

We made a restriction analysis and sequenced parts of this BioBrick.


<partinfo>P1010</partinfo>: ccdB-gene

We transformed this BioBrick into Escherichia coli JM109, DH5α, TOP10 and DB3.1. E. coli JM109 and DH5α seem to be ccdB resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in E. coli TOP10 (no colonies after transformation) and DB3.1 (lawn after transformation).


Table 1: Results of the transformation of the cell-death gene ccdB, BioBrick <partinfo>P1010</partinfo>, into different strains of E. coli.

E. coli strain Resistant to <partinfo>P1010</partinfo>? Expected result?
DB3.1 yes yes
DH5α yes no
JM109 yes no
TOP10 no yes


<partinfo>K389011</partinfo>: VirA screening device

Ratio of surviving colonies of E. coli EC100D carrying unmutated <partinfo>K389010</partinfo> and <partinfo>K389011</partinfo> plated on PA agar plates with chloramphenicol, ampicillin and different concentrations of kanamycin. Comparison between cells that were induced with acetosyringone with cells that were not induced.


Sequenced BioBricks

Own BioBricks

The sequencing of the following of our own BioBricks was succesful and lead to the expected results:

  • <partinfo>K389001</partinfo> (not fully completed)
  • <partinfo>K389002</partinfo>
  • <partinfo>K389003</partinfo>
  • <partinfo>K389004</partinfo>
  • <partinfo>K389005</partinfo>
  • <partinfo>K389010</partinfo> (not fully completed)
  • <partinfo>K389011</partinfo> (not fully completed)
  • <partinfo>K389012</partinfo> (not fully completed)
  • <partinfo>K389013</partinfo> (not fully completed)
  • <partinfo>K389015</partinfo> (not fully completed)
  • <partinfo>K389016</partinfo> (not fully completed)
  • <partinfo>K389050</partinfo>


Other BioBricks

  • <partinfo>K238008</partinfo> sequencing gave negative results - infos in registry are not correct!
  • <partinfo>K238011</partinfo> sequencing gave negative results - infos in registry are not correct!
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